Hi-SYBr Master Mix (with Taq Polymerase), supplied in 2X concentration, is a ready-to-use mix convenient for realtime PCR. The master mix contains:
1.SYBr Green Dye
2.Taq polymerase
3.dNTPs
4.Assay buffer
5.MgCl2
Template, primers and nuclease-free water should be added before setting up the PCR reaction. As the mixture is ready-to-use, the reaction set time is reduced to half.