HiPurA Total RNA Miniprep Purification Kit provides a fast and easy method for purification of total RNA using the miniprep spin columns. This kit simplifies isolation of RNA from animal cells, animal tissues and yeast, and for cleanup of RNA from crude enzymatic reactions (e.g. DNase digestion, Proteinase digestion, RNA ligation, and labeling reaction). It is based on the advanced silica binding principle presented in a microspin format facilitating the binding, washing and elution steps thus enabling multiple samples to be processed simultaneously. It comprises of three steps viz, adsorption of RNA to the membrane, removal of residual contaminants and elution of pure RNA. Concentration of recovered RNA can be estimated by agarose gel electrophoresis. Typical yield is upto 100µg from 107 cells, 15 - 30mg animal tissue.