HiPurA® Viral DNA/ RNA Purification Kit provides a fast and easy method for purification of total DNA/ RNA from a wide variety of DNA and RNA viruses. The DNA and RNA purification procedure using the miniprep spin-column comprises of three steps viz. adsorption of DNA/
RNA to the membrane, removal of residual contaminants and elution of pure Viral DNA/ RNA. HiMedia’s HiElute Miniprep spin-column (Capped) format allows rapid processing of multiple samples. The columns have a high binding capacity and high-quality Viral DNA/ RNA is obtained from various species.
This kit simplifies isolation of DNA/ RNA from fresh, old (more than 24 hours) and frozen plasma, serum and other body fluids with spin-column procedure. Viral DNA/ RNA purification involves cell lysis, which is achieved by incubation of sample in a solution containing chaotropic ions in the presence of Proteinase K at 56o C. HiElute Miniprep spin-column (Capped) contains specially developed membranes for optimal binding of Viral DNA and RNA. After the initial binding of DNA/ RNA, impurities like proteins, polysaccharides, low molecular weight metabolites and salts are removed by short washing steps. high-quality DNA/ RNA is finally eluted in the Elution Buffer provided with the kit.