For screening Cronobacter (formerly Enterobacter sakazakii) from food. The composition and performance of this media are as per specifications laid down in ISO 22964:2017(E).
Cronobacter Selective Broth (CSB) is recommended for screening Cronobacter (formerly Enterobacter sakazakii) from food. The composition and performance of this media are as per specifications laid down in ISO 22964:2017(E). It contains Peptone and HM extract that provide carbonaceous, nitrogenous compounds, long chain amino acids and other essential nutrients. Sodium chloride maintains osmotic equilibrium. Sucrose present is fermented by Cronobacter. Consequently the broth turns yellow after incubation.
1. Mullane et al. 2007.Minerva Pediatr. 59.137-148.2.Lai.2001.Medicine.80.113-122.3.Iversen et al.2008.Appl.Environ.Microbiol.74, 2550-2552.4.Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition.5.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1.6.American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., WashingtonD.C.7.Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2001, Compendium of Methods for the Microbiological Examination ofFoods, 5th Ed., American Public Health Association, Washington, D.C.8.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,APHA Inc., Washington, D.C.