Calcein AM is a non-fluorescent, hydrophobic compound that is easily permeable in intact and live cells. It is used to determine cell viability in most eukaryotic cells. The signal (excitation and emission of 488nm / 520nm respectively) of Calcein-AM is proportional to cell viability, as esterase activity decreases in cells with poor viability.
Calcein AM is a non-fluorescent, hydrophobic compound that is easily permeable in intact and live cells. It is used to determine cell viability in most eukaryotic cells. Calcein AM is converted to a green-fluorescent calcein after acetoxymethyl ester hydrolysis by intracellular esterases, which is retained in the cytoplasm. The signal (excitation and emission of 488nm / 520nm respectively) of Calcein-AM is proportional to cell viability, as esterase activity decreases in cells with poor viability.