Tween 20 is a non-ionic detergent widely used in the biochemical applications. It serves as an emulsifying agent and is widely used for the preparation of stable oil-in-water emulsions, particularly in pharmaceutical applications. To remove the peripheral proteins, tween-20 has been initially used in the pre-extraction of membranes. It also serves as a blocking agent on nitrocellulose.
Quality Control
Appearance
Pale yellow to brownish yellow viscous, oily liquid
Solubility
1 mL miscible in 1 mL of water
pH (5% in water at 25°C)
5.00-7.00
Refractive index (n 20/D)
1.47 -1.47
Hydroxyl value
96.00-108.00
Saponification value
40.00 -50.00
Acid value
0.00-2.00
Iodine value
0.00-5.00
Sulphated ash
0.00 -0.20
Water (K.F.)
0.00-3.00
Cell Culture Test
Passes
Storage and Shelf Life
Storage Condition
Store below 30°C.
Shelf Life Instruction
Use before expiry date given on the product label.