Triton® X-100

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SKU:
TC286
CAS Number : 9002-93-1
Synonym : octyl phenol ethoxylate
Molecular Formula : C₃₄H₆₂O₁₁
Triton® X 100 is a non-ionic, non-denaturing mild detergent with a bulky non-polar head group. In biochemical applications, Triton® X 100 is used to control protein crystallization, to prevent non-specific binding in affinity purification and in immunoassay procedures and as an additive in electrophoresis buffers.
CAS Number : 9002-93-1
Synonym : octyl phenol ethoxylate
Molecular Formula : C₃₄H₆₂O₁₁


In cell culture, Triton® X 100 is used for multiple applications such as lysis of cells and intracellular organelles, solubilization of membrane proteins and lipids, and membrane permeabilization during transfection procedures. It is used typically at a concentration of 0.1% to 0.5% for lysing cells.

Although Triton® X 100 is considered as a mild detergent, it can be cytotoxic to cells if used in excess amount or, if cells are exposed to it for prolonged duration. Toxicity of Triton® X-100 is attributed to disrupting action of its polar head group on hydrogen bonding present within the cell’s lipid bilayer.

CAS Number : 9002-93-1
Synonym : octyl phenol ethoxylate
Molecular Formula : C₃₄H₆₂O₁₁
More Information
Product Name Triton® X-100
SKU TC286
CAS No 9002-93-1
Molecular formula C₃₄H₆₂O₁₁
Synonym octyl phenol ethoxylate
Storage Below 30°C
Shelf Life 4 Years
Customized Product Available No
Appearance Colorless to light yellow slightly hazy liquid.
Solubility Clear colorless solution at 5ml in 100ml of water.
pH (5% in water at 20°C) 6.00 - 8.00
FTIR (Liquid film) Matches with the standard pattern
Density (at 25°C) ~1.06 g/mL
Refractive index (n 20/D) 1.490 - 1.492
Water (K.F.) <= 1.00%
Hydroxyl value 85 - 101
Residue on ignition <= 0.40%
Cell Culture Test Passes
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