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Triton® X-100
SKU:
TC286
CAS Number :
9002-93-1
Synonym :
octyl phenol ethoxylate
Molecular Formula :
C₃₄H₆₂O₁₁
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Triton® X 100 is a non-ionic, non-denaturing mild
detergent with a bulky non-polar head group.
In biochemical applications, Triton® X 100 is used to
control protein crystallization, to prevent non-specific
binding in affinity purification and in immunoassay
procedures and as an additive in electrophoresis buffers.
CAS Number :
9002-93-1
Synonym :
octyl phenol ethoxylate
Molecular Formula :
C₃₄H₆₂O₁₁
Product Description
| Molecular weight | 647 |
|---|---|
| CAS No. | 9002-93-1 |
| Synonyms | octyl phenol ethoxylate, polyethylene glycol p- (1,1,3,3-tetramethylbutyl)-phenyl ether, polyoxyethylene octyl phenyl ether, 4-octylphenol polyethoxylate, TX-100 |
Triton® X 100 is a non-ionic, non-denaturing mild detergent with a bulky non-polar head group.
In biochemical applications, Triton® X 100 is used to control protein crystallization, to prevent non-specific binding in affinity purification and in immunoassay procedures and as an additive in electrophoresis buffers.
In cell culture, Triton® X 100 is used for multiple applications such as lysis of cells and intracellular organelles, solubilization of membrane proteins and lipids, and membrane permeabilization during transfection procedures. It is used typically at a concentration of 0.1% to 0.5% for lysing cells.
Although Triton® X 100 is considered as a mild detergent, it can be cytotoxic to cells if used in excess amount or, if cells are exposed to it for prolonged duration. Toxicity of Triton® X-100 is attributed to disrupting action of its polar head group on hydrogen bonding present within the cell's lipid bilayer.
Directions:
Preparation instructions:
Triton® X-100 is soluble in water, benzene, toluene, xylene, trichloroethylene, ethylene glycol, ethyl ether, ethanol, isopropanol, and ethylene dichloride.
Solutions of Triton® X 100 can be sterilized by filtration or by autoclaving. Cloudiness may be observed after autoclaving which clears with stirring of the cooled solution.
Quality Control:
| Appearance | Colorless to light yellow slightly hazy liquid. |
|---|---|
| Solubility | Clear colorless solution at 5ml in 100ml of water. |
| pH (5% in water at 20°C) | 6.00-8.00 |
| FTIR (Liquid film) | Matches with the standard pattern |
| Density (at 25°C) | ~1.06 g/mL |
| Refractive index (n 20/D) | 1.490 - 1.492 |
| Water (K.F.) | <= 1.00% |
| Hydroxyl value | 85-101 |
| Residue on ignition | <= 0.40% |
| Cell Culture Test | Passes |
Storage and Shelf Life:
| Storage conditions | Store powder below 30°C in air tight container. |
|---|---|
| Shelf life | 48 months. |
| Use before | expiry date given on product label. |
CAS Number :
9002-93-1
Synonym :
octyl phenol ethoxylate
Molecular Formula :
C₃₄H₆₂O₁₁
| Product Name | Triton® X-100 |
|---|---|
| SKU | TC286 |
| CAS No | 9002-93-1 |
| Molecular formula | C₃₄H₆₂O₁₁ |
| Synonym | octyl phenol ethoxylate |
| Storage | Below 30°C |
| Shelf Life | 4 Years |
| Appearance | Colorless to light yellow slightly hazy liquid. |
|---|---|
| Solubility | Clear colorless solution at 5ml in 100ml of water. |
| pH (5% in water at 20°C) | 6.00 - 8.00 |
| FTIR (Liquid film) | Matches with the standard pattern |
| Density (at 25°C) | ~1.06 g/mL |
| Refractive index (n 20/D) | 1.490 - 1.492 |
| Water (K.F.) | <= 1.00% |
| Hydroxyl value | 85 - 101 |
| Residue on ignition | <= 0.40% |
| Cell Culture Test | Passes |
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