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L-Histidine monohydrochloride monohydrate
SKU:
TC077M
CAS Number :
5934-29-2
Molecular Formula :
C₆H₉N₃O₂.HCl.H₂O
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Product Code
| Product Code | TC077M |
|---|
Product Description
| Molecular weight | 209.63 |
|---|---|
| Molecular formula | C6H9N3O2.HCl.H2O |
| CAS No. | 5934-29-2 |
Quality Control
| Appearance (EP, BP) | White or almost white, crystalline powder or colourless crystals |
|---|---|
| Appearance (JP) | White crystals or a white crystalline powder |
| Solubility (EP, BP) | Freely soluble in water, slightly soluble in ethanol (96%) |
| Solubility (JP) | It is freely soluble in water and practically insoluble in ethanol (99.5%). It dissolves in 6 mol/L hydrochloric acid. |
| Identification 1, FTIR (JP) | Matches with the standard pattern |
| Identification A, Specific rotation (EP, BP) | +9.20 to +10.60° (c = 2.75g in 12 mL of 6M HCl and dilute to 25.0 mL with water, at 20 ± 0.5°C, dried substance) |
| Identification 2 (JP) | Reactions for chlorides |
| Identification B, pH (EP, BP) | 3.00 - 5.00 (c = 5% in water at 25°C) |
| Identification C, FTIR (EP, BP) | Matches with the standard pattern |
| Identification D, TLC (EP, BP) | The principal spot in the chromatogram obtained with the test solution is similar in position, colour and size to the principle spot in the chromatogram obtained with the reference solution |
| Identification E (EP, BP) | An orange colour develops |
| Identification F (EP, BP) | Reactions for chlorides |
| Appearance of solution (EP, BP) | Solution is clear and not more intensely coloured than reference solution BY6 |
| Clarity and colour of solution (JP) | 10% solution in water is clear and colourless |
| pH (5% in water at 25°C) (EP, BP) | 3.00-5.00 |
| pH (10% in water at 25°C) (JP) | 3.50-4.50 |
| Ammonium (Amino acid analysis) (EP, BP) | Complies |
| Ammonium (JP) | <= 0.02% |
| Sulfates (EP, BP) | <= 0.03% |
| Sulfates (JP) | <= 0.028% |
| Iron (EP, BP, JP) | <= 0.001% |
| Heavy metals (JP) | <= 0.001% |
| Specific rotation (EP, BP) | +9.20 to +10.60° (c = 2.75g in 12 mL of 6M HCl and dilute to 25.0 mL with water, at 20 ± 0.5°C, dried substance) |
| Specific rotation (JP) | +9.20 to +10.60° (c = 11.0% in 6M HCl, on anhydrous basis, at 25°C) |
| Releted substances : TLC (JP) | The spot other than the principal spot obtained from the sample solution is not more intense than the spot obtained from the standard solution |
| Ninhydrin-positive substances : System suitability : Resolution (EP, BP) | min. 1.5 between the peaks due to isoleucine and leucine |
| Ninhydrin-positive substances (Amino acid analysis - for each impurity) (EP, BP) | <= 0.20% |
| Ninhydrin-positive substances (Amino acid analysis - total impurity) (EP, BP) | <= 0.5% |
| Sulfated ash (EP, BP) | <= 0.10% |
| Residue on ignition (JP) | <= 0.10% |
| Loss on drying (EP, BP) | 7.00 - 10.00% (at 145 - 150°C, 2hr) |
| Water (JP) | 7.20-10.00% |
| Assay (NaOH Titration, on dry basis) (EP, BP) | 98.50 - 101.00% |
| Assay (NT, anhydrous basis) (JP) | 99.00 - 101.00% |
Storage and Shelf Life
| Storage | Store below 30°C away from bright light. |
|---|---|
| Shelf life | 48 months. |
| Usage | Use before expiry date given on the product label. |
CAS Number :
5934-29-2
Molecular Formula :
C₆H₉N₃O₂.HCl.H₂O
| Product Name | L-Histidine monohydrochloride monohydrate |
|---|---|
| SKU | TC077M |
| CAS No | 5934-29-2 |
| EC No. | 211-438-9 |
| Molecular formula | C₆H₉N₃O₂.HCl.H₂O |
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