Spectinomycin dihydrochloride pentahydrate is an
aminocyclitol antibiotic which is closely related to
the aminoglycosides, produced by a species of soil
microorganism Streptomyces spectabilis. It has a broad
spectrum of activity against many Gram-positive and
Gram-negative bacteria.
Spectinomycin dihydrochloride pentahydrate is an aminocyclitol antibiotic which is closely related to the aminoglycosides, produced by a species of soil microorganism Streptomyces spectabilis. It has a broad spectrum of activity against mangy Gram-positive and Gram-negative bacteria. It exerts bactericidal effect by binding to 30S ribosomal subunit and interfering with the translocation of peptidyl tRNA, thus inhibiting protein synthesis. In cell culture, it is used as an anti-bacterial agent to prevent contamination.
Preparation instructions
For cell culture applications, Spectinomycin dihydrochloride pentahydrate solutions can be prepared as per required concentrations in water. Solutions can be sterilized by filtering through a sterile membrane filter with a porosity of 0.22 microns or less.
Appearance
White to pale buff crystals or powder.
Solubility
100 mg soluble in 1 ml of water.
pH (1% in water at 25°C)
3.80 - 5.60
Water (K.F)
16.00 - 20.00%
Potency
NLT 603 µg/mg
Antibiotic sensitivity
Passes
Cell Culture Test
Passes
Storage
Store powder at 2 - 8°C in air tight containers and away from bright light. Use before expiry date given on the product label.
Store spectinomycin dihydrochloride pentahydrate powder at 2°C to 8°C. Stock solutions should be stored at 2°C to 8°C for several weeks. Their stability can be extended by storing at -20°C.