Erythromycin

Product Identifier

Description

Erythromycin is a macrolide antibiotic produced by Streptomyces erythreus. It is a broad spectrum antibiotic with bacteriostatic action against a wide range of Gram positive and to a lesser extent some Gram negative bacteria, Mycoplasma spp, Chlamydia, Rickettsia spp. and Spirochetes.

Erythromycin inhibits protein synthesis by binding reversibly to the 50S subunit of the ribosome, resulting in blockage of the transpeptidation or translocation reactions. Erythromycin interferes with aminoacyl translocation, preventing the transfer of tRNA bound at the A site to the P site of rRNA complex.

In cell culture, Erythromycin is used as an antibacterial agent to prevent contamination.

Directions

Preparation instructions:

For cell culture applications, Erythromycin solutions can be prepared as per required concentrations in water, ethanol and 2N hydrochloric acid.

Solutions can be sterilized by filtering through a sterile membrane filter with porosity of 0.22 microns or less.

Quality Control

Storage and Shelf Life

• Store below 30°C in air tight containers and away from bright light.
• Store the stock solution of Erythromycin at 2 - 8°C.
• Shelf life is 36 months.
• Use before expiry date given on the product label.