Trypsin is a serine protease derived from porcine pancreas. Ethylenediaminetetraacetic acid (EDTA), a chelating agent is often added to enhance enzymatic activity of trypsin solution. This product contains 0.25% Trypsin and 0.2% EDTA in Dulbecco’s phosphate buffered saline.
Technical Data Sheet (TD)
Trypsin-EDTA Solution 1X 0.25% Trypsin and 0.2% EDTA in Dulbecco's Phosphate Buffered Saline w/o Phenol red
Safety Data Sheet (SDS)
Trypsin-EDTA Solution 1X 0.25% Trypsin and 0.2% EDTA in Dulbecco's Phosphate Buffered Saline w/o Phenol red
Electronic Instructions for Use (eIFU)
Trypsin-EDTA Solution 1X 0.25% Trypsin and 0.2% EDTA in Dulbecco's Phosphate Buffered Saline w/o Phenol red
Trypsin-EDTA Solution 1X 0.25% Trypsin and 0.2% EDTA in Dulbecco's Phosphate Buffered Saline w/o Phenol red
Trypsin is a single chain polypeptide of 223 amino acid residue with substrate specificity based on positively charged Lysine and Arginine side chains. Trypsin predominantly cleaves peptide chains at the carboxyl sides of Lysine and Arginine, except when either is followed by Proline. It is most commonly used for dissociation and disaggregation of adherent cells. EDTA acts by neutralizing calcium and magnesium ions that enhance cell to cell adhesion. Trypsin is a serine protease derived from porcine pancreas. Ethylenediaminetetraacetic acid (EDTA), a chelating agent is often added to enhance enzymatic activity of trypsin solution. This product contains 0.25% Trypsin and 0.2% EDTA in Dulbecco’s phosphate buffered saline.