Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Intended Use
For determining efficiency of sanitization of containers, equipments, surfaces, water miscible cosmetics etc.
Composition
| Ingredients | Gms/Litre |
|---|---|
| Tryptone | 15.000 |
| Soya peptone | 5.000 |
| Sodium chloride | 5.000 |
| Lecithin | 5.000 |
| Polysorbate 80 | 20.000 |
| Glycerol | 10.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.3±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
SCDM w/0.5% Lecithin, 2% Polysorbate 80 and 1% Glycerol is used in RODAC (Replicate Organism Detection and Counting) plates (1) for the detection and enumeration of microorganisms present on surfaces of sanitary importances (2,3).
Tryptone and Soya peptone provide nitrogenous and carbonaceous compounds, long chain amino acids, vitamins and other nutrients essential for microbial replication. Lecithin to inactivate residual disinfectants from where the sample is collected (4). Lecithin neutralizes quaternary ammonium compounds. Polysorbate 80 neutralizes phenolic disinfectants, hexachlorophene, formalin and with lecithin ethanol (5). Glycerol helps in retention of moisture and serves as carbon source.
Collection of samples from areas before and after the treatment with disinfectant evaluates cleaning procedures in environmental sanitation.
The presence and number of microorganisms is determined by the appearance of colonies on the agar surface (6). After counting the colonies, carry out biochemical carry out biochemical testing for identification.
Quality Control
Appearance:
Sterile Tryptone Soya Agar Plate w/ Lecithin & Polysorbate 80 in 55 mm scored disposable plates with convex surface and absence of black particles/ cracks/ bubbles.
Colour:
Light yellow coloured medium.
Quantity of Medium:
20 ml of medium in 55 mm scored plates
pH:
7.10-7.50
Dose of irradiation:
10.00-25.00
Cultural Response:
Growth Promotion was observed after an incubation at 30-35°C for 18-24 hours.
Recovery rate:
Recovery rate is considered 100% for bacteria growth on Soyabean Casein Digest Agar.
Growth of microorganism comparable to that previously obtained with previously tested and approved lot of medium occurs at the specified temperature for not more than the shortest period of time specified inoculating <=100 cfu (at 30-35°C for 18 hours).
Sterility Test
Passes release criteria
Cultural Response
| Organism | Inoculum (CFU) | Growth | Observed Lot value (CFU) | Recovery | Incubation temperature | Incubation period |
|---|---|---|---|---|---|---|
| Staphylococcus aureus ATCC 25923 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Staphylococcus aureus ATCC 6538 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Escherichia coli ATCC 25922 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Escherichia coli ATCC 8739 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Pseudomonas aeruginosa ATCC 27853 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Pseudomonas aeruginosa ATCC 9027 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
Storage and Shelf Life
Store between 20-30°C. Use before expiry date on the label.
| Product Name | Soyabean Casein Digest Agar Plate w/0.5% Lecithin, 2% Polysorbate 80 and 1% Glycerol (γ-irradiated) (Triple pack) |
|---|---|
| SKU | SP5297GT |
| Customized Product Available | No |
Write Your Own Review