Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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A general purpose medium for cultivation of wide variety of microorganisms.
Composition**
| Ingredients | Gms/Litre |
|---|---|
| Pancreatic digest of casein | 15.000 |
| Papaic digest of soyabean meal | 5.000 |
| Sodium chloride | 5.000 |
| Agar | 15.000 |
**Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100CFU) aseptically on the plate.
Principle And Interpretation
Soyabean Casein Digest Agar is a widely used medium, which supports the growth of wide variety of organisms even that of fastidious ones such as Neisseria, Listeria, and Brucella etc. The medium with addition of blood provides perfectly defined haemolysis zones, while preventing the lysis of erythrocytes due to its sodium chloride content.
It has been frequently used in the health industry to produce antigens, toxins etc. Its simple and inhibitor-free composition makes it suitable for the detection of antimicrobial agents in the food and other products. Tryptone Soya Agar is recommended by various pharmacopoeias as sterility testing medium (1, 2).
Tryptone Soya Agar conforms as per USP (1) and is used in microbial limit test and antimicrobial preservative - effective test. Gunn et al (3) used this medium for the growth of fastidious organisms and study of haemolytic reaction after addition of 5% v/v blood. The combination of Pancreatic digest of casein and papaic digest of soyabean meal makes this media nutritious by providing amino acids and long chain peptides for the growth of microorganisms. Sodium chloride maintains the osmotic balance.
Soyabean Casein Digest Agar does not contains X and V growth factors. It can be conveniently used in determining the requirements of these growth factors by isolates of Haemophilus by the addition of X-factor (DD020), V-factor (DD021), and X+V factor discs (DD022) factor to inoculated TSA plates (4).
Quality Control
Appearance
Sterile Soyabean Casein Digest Agar in scored plates
Colour
Light yellow coloured medium.
Quantity of Medium
28ml of medium in scored plates
pH
7.10-7.50
Cultural Response
Recovery rate is considered 100% for bacteria growth on Blood Agar and fungus growth on Sabouraud Dextrose Agar.
Growth promoting properties
Growth of microorganism comparable to that previously obtained with previously tested and approved lot of medium occurs at the specified temperature for not more than the shortest period of time specified inoculating <=100 cfu (at 30-35°C for 18 hours).
Sterility Test
Passes release criteria.
Cultural Response (SP290A)
SP290A: Growth Promotion was carried out in accordance with the harmonized method and growth was observed after an incubation at 30-35°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Observed Lot value (CFU) | Recovery | Incubation temperature | Incubation period |
|---|---|---|---|---|---|---|
| Bacillus subtilis ATCC 6633 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Staphylococcus aureus ATCC 25923 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Staphylococcus aureus ATCC 6538 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Escherichia coli ATCC 25922 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Escherichia coli ATCC 8739 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Escherichia coli NCTC 9002 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Pseudomonas aeruginosa ATCC 27853 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Pseudomonas aeruginosa ATCC 9027 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Salmonella Abony NCTC 6017 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Micrococcus luteus ATCC 9341 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Streptococcus pneumoniae ATCC 6305 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Salmonella Typhimurium ATCC 14028 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Candida albicans ATCC 10231 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | <=5 d |
| Candida albicans ATCC 2091 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | <=5 d |
| Aspergillus brasiliensis ATCC 16404 | 50-100 | Good-luxuriant | 25 -70 | 50-70% | 30-35 °C | <=5 d |
| Aspergillus brasiliensis ATCC 16404 | 50-100 | Luxuriant | 35-100 | >=70% | 20-25 °C | <=5 d |
Storage and Shelf Life
Store between 20-30°C. Use before expiry date on the label.
| Product Name | Soyabean Casein Digest Agar Plate (Tryptone Soya Agar Plate) (75mm scored Petri plate) |
|---|---|
| SKU | SP290A |
| Customized Product Available | No |
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