Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Intended use
Recommended for determining efficiency of sanitization of containers, equipment surfaces, water miscible cosmetics etc. It can also be used to enumerate the organisms from water insoluble products & fatty products containing preservatives or antimicrobials.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Tryptone | 15.000 |
| Soya peptone | 5.000 |
| Yeast extract | 6.000 |
| Sodium pyruvate | 2.000 |
| Sodium chloride | 5.000 |
| Soya Lecithin | 0.700 |
| Polysorbate 80 (Tween 80) | 5.000 |
| Histidine | 1.000 |
| Sodium thiosulphate pentahydrate | 0.050 |
| Agar | 20.500 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
SCDA Plate w/sodium pyruvate, yeast extract & LTHTh, Modified is used for the detection and enumeration of microorganisms for products of sanitary importance, water miscible cosmetics, products containing antimicrobials or preservatives (1).
Tryptone, soya peptone, yeast extract and sodium pyruvate provide nitrogenous, carbonaceous compounds, long chain amino acids, vitamins and other nutrients essential for microbial replication. Lecithin, polysorbate 80 (Tween 80) and thiosulphate act as neutralizing agents reported to neutralize the activity of antimicrobial agents. Lecithin and polysorbate 80 neutralizes quaternary ammonium compounds and parahydroxy benzoates. Sodium thiosulphate neutralizes mercurial, halogens, aldehydes etc. Histidine acts as a reducing agent.
Type of specimen
Environmental monitoring samples
Specimen Collection and Handling
Collection of samples from areas before and after the treatment with disinfectant evaluates cleaning procedures in environmental sanitation. The presence and number of microorganisms is determined by the appearance of colonies on the agar surface (2).
Warning and Precautions
Read the label before opening the pack. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual strain of a microorganism may have unique growth requirements with respect to nutrients and physical conditions. Based on which the growth pattern of each varies on a medium and some even may display significant delay in development.
- Environmental Monitoring Test: Exposure of media plates for 4 has a settle plate or in air sampler or even under laminar air flow may lead reduction in some available moisture on the surface. This may cause development of tiny cracks in the agar or slight shrinkage. This however, does not impact the performance of the media.
- Further biochemical identification is necessary for confirmation.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile SCDA Plate w/sodium pyruvate, yeast extract & LTHTh in 55mm plates with smooth surface and absence of black particles/cracks/bubbles (y-irradiated, Triple pack)
Colour
Light yellow coloured medium.
Quantity of Medium
20ml of medium in 55mm plates
pH
7.10-7.50
Dose of irradiation
13.00-20.00
Sterility Check
Passes release criteria.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Growth | Growth w/ disinfectant |
|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | luxuriant | fair-good, (depends on concentration of quarternary ammonium compounds) |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | luxuriant | fair-good, (depends on concentration of quarternary ammonium compounds) |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | luxuriant | fair-good, (depends on concentration of quarternary ammonium compounds) |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 20-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | SCDA Plate w/sodium pyruvate, yeast extract & LTHTh, Modified (γ-irradiated)(Triple pack) |
|---|---|
| SKU | SP2113GT |
| Customized Product Available | No |
Write Your Own Review