Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended use
Recommended as a general purpose medium for cultivation of wide variety of organisms and for inactivation of ẞ lactam antibiotics.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Tryptone # | 15.000 |
| Soya peptone | 5.000 |
| Sodium chloride | 5.000 |
| Agar | 15.000 |
| ẞ Lactamase | 330 IU/lit |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Pancreatic digest of casein
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Tryptone Soya Agar with ẞ Lactamase is used in plates for the detection and enumeration of microorganisms present on surfaces of sanitary importances and also in environmental monitoring of clean room for facilities where production of ẞ-lactam antibiotics are carried out. Tryptone and soya peptone provide nitrogenous compounds and other nutrients essential for microbial replication. ẞ Lactamase inactivates ẞ-lactam antibiotics, thus enabling the growth of resistant strains present in the environment of clean rooms where production of antibiotics is carried out.
Type of specimen
Pharmaceutical samples
Specimen Collection and Handling
For Pharmaceutical samples follow appropriate techniques for sample collection, handling and processing as per pharmacopoeias. After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred to individual safety data sheets.
Limitations
- Individual strain of a microorganism shows different growth pattern due to their unique growth requirements with respect to nutrients and physical conditions.
- Environmental Monitoring Test: Exposure of media plates for 4 h as a settle plate or in air sampler or even under laminar air flow may lead reduction in some available moisture on the surface. This may cause development of tiny cracks in the agar or slight shrinkage. This however, does not impact the performance of the media.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile Soyabean Casein Digest Agar w/ ẞ-lactamase I in 55mm scored disposable plates with convex surface and absence of black particles/cracks/bubbles (y irradiated, Triple Pack).
Colour
Light yellow coloured medium.
Quantity of Medium
20ml of medium in 55 mm scored disposable plate.
Sterility Check
Passes release criteria
pH
7.10-7.50
Dose of Irradiation (Kgy)
13.00-20.00
Concentration
Concentration of Penicillinase added: 330 IU/lit of media (approximately 2,00,000 LU/lit)
Cultural Response
Growth Promotion Test of as such plates was carried out and growth was observed after incubation at 30-35°C for <= 3 days. Simultaneously growth promotion test was carried out on plates which were seeded with 100 mcg/ml of Benzyl Penicillin.
Recovery rate is considered 100% for bacteria growth on Soyabean Casein Digest Agar.
| Organism | Inoculum (CFU) | Growth | Recovery | Growth w/ antibiotic | Recovery w/ antibiotic |
|---|---|---|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | 50-100 | Luxuriant | >=70% | Luxuriant | >=70% |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | Luxuriant | >=70% | Luxuriant | >=70% |
| Staphylococcus aureus subsp. aureus ATCC 29213 (00131*) | 50-100 | Luxuriant | >=70% | Luxuriant | >=70% |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | Luxuriant | >=70% | Luxuriant | >=70% |
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | Luxuriant | >=70% | Luxuriant | >=70% |
| Proteus mirabilis ATCC 25933 | 50-100 | Luxuriant | >=70% | Luxuriant | >=70% |
Key :(*) - Corresponding WDCM numbers
Storage and Shelf Life
On receipt store between 20-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (1,2).
| Product Name | Soyabean Casein Digest Agar Plate w/ β-Lactamase (Tryptone Soya Agar Plate w/ β-Lactamase) (γ irradiated) (Triple Pack) |
|---|---|
| SKU | SP1765GT |
| Customized Product Available | No |
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