Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for determining the efficiency of sanitization of containers, equipment surfaces, water miscibl cosmetics,etc. It can also be used to enumerate the organisms from water insoluble products and fatty products containing preservatives or antimicrobials.
Composition
| Ingredients | g/L |
|---|---|
| Tryptone | 15.000 |
| Soya peptone | 5.000 |
| Sodium chloride | 5.000 |
| Lecithin | 0.700 |
| Polysorbate 80 (Tween 80) | 5.000 |
| Histidine | 0.500 |
| Sodium thiosulphate | 0.500 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.3±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Soyabean Casein Digest Agar w/ LTHTh is used for the detection and enumeration of microorganisms for products of sanitary importance, water miscible cosmetics, products containing antimicrobials or preservatives (1).
Tryptone and soya peptone provide nitrogenous compounds and other nutrients essential for microbial replication. Lecithin, polysorbate 80 (Tween 80) and thiosulphate act as neutralizing agents reported to neutralize the activity of antimicrobial agents. Lecithin and polysorbate 80 neutralizes quaternary ammonium compounds and parahydroxy benzoates. Sodium thiosulphate neutralizes mercurial, halogens, aldehydes etc. Histidine acts as a reducing agent.
Type of specimen
Environmental samples.
Specimen Collection and Handling
Collection of samples from areas before and after the treatment with disinfectant evaluates cleaning procedures in environmental sanitation. The presence and number of microorganisms is determined by the appearance of colonies on the agar surface (2,3).
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- This medium is general purpose medium and may not support the growth of fastidious organisms.
- Further biochemical and serological test must be carried out for complete identification.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile Soyabean Casein Digest Agar Plate w/LTHTh in 55mm scored disposable plates with convex surface and absence of black particles/ cracks/ bubbles.(y-irradiated, Triple pack)
Colour: Light to medium amber coloured medium.
Quantity of Medium: 20ml of medium in 55 mm scored plate.
pH: 7.10-7.50
Dose of irradiation: 13.00-20.00
Sterility Check: Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation for bacterial cultures at 30-35°C for 18-24 hours and for fungal cultures at 20-25°C & 30-35°C for <=5 days.
Recovery rate
Recovery is considered 100% for bacterial growth on Soyabean Casein Digest Agar and fungal growth on Sabouraud Dextrose Agar.
Growth promoting properties
Growth of microorganism comparable to that previously obtained with previously tested and approved lot of medium occurs at the specified temperature for not more than the shortest period of time specified inoculating <=100 cfu(at 30-35°C for 18-24 hours) for bacteria. Growth is also observed at 20-25°C for <=5 days for fungus.
Neutralization Test
The smaller zone of inhibition compared to SCDA indicates neutralization of quarternary ammonium compounds by this medium.
| Organism | Inoculum (CFU) | Growth | Recovery | Incubation temperature | Incubation period |
|---|---|---|---|---|---|
| **Bacillus spizizenii ATCC 6633 (00003*) | 50-100 | luxuriant | >=70% | 30-35 °C | 18-24 hrs |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant | >=70% | 30-35 °C | 18-24 hrs |
| Staphylococcus aureus subsp. aureus ATCC 6538 (00032*) | 50-100 | luxuriant | >=70% | 30-35 °C | 18-24 hrs |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | >=70% | 30-35 °C | 18-24 hrs |
| Escherichia coli ATCC 8739 (00012*) | 50-100 | luxuriant | >=70% | 30-35 °C | 18-24 hrs |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | luxuriant | >=70% | 30-35 °C | 18-24 hrs |
| ^Pseudomonas paraeruginosa ATCC 9027 (00026*) | 50-100 | luxuriant | >=70% | 30-35 °C | 18-24 hrs |
| $Kocuria rhizophila ATCC 9341 | 50-100 | luxuriant | >=70% | 30-35 °C | 18-24 hrs |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant | >=70% | 30-35 °C | 18-24 hrs |
| Salmonella Abony NCTC 6017 (00029*) | 50-100 | luxuriant | >=70% | 30-35 °C | 18-24 hrs |
| Candida albicans ATCC 2091 (00055*) | 50-100 | luxuriant | >=70% | 30-35 °C | 18-24 hrs |
| Candida albicans ATCC 10231 (00054*) | 50-100 | luxuriant | >=70% | 30-35 °C | 18-24 hrs |
| #Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | luxuriant | >=70% | 30-35 °C | 18-24 hrs |
| Candida albicans ATCC 2091 (00055*) | 50-100 | luxuriant | >=70% | 20-25 °C | <=3 d |
| Candida albicans ATCC 10231 (00054*) | 50-100 | luxuriant | >=70% | 20-25 °C | <=3 d |
| #Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | luxuriant | >=70% | 20-25 °C | <=3 d |
| Clostridium sporogenes ATCC 19404 (00008*) | 50-100 | luxuriant | >=70% | 30-35 °C | 48hrs |
Key:
- (*) Corresponding WDCM numbers,
- ^ Formerly known as Pseudomonas aeruginosa
- # Formerly known as Aspergillus niger
- ** Formerly known as Bacillus subtilis subsp. spizizenii
- $ Formerly known as Micrococcus luteus
Storage and Shelf Life
On receipt store between 20-30°C Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | Soyabean Casein Digest Agar Plate w/LTHTh (Tryptone Soya Agar Plate w/LTHTh) (γ-irradiated) (Triple pack) |
|---|---|
| SKU | SP1691GT |
| Customized Product Available | No |
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