Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Soyabean Casein Digest Agar w/ 1% Soya lecithin and 4% Polysorbate 80
Sterility test medium also used for cultivation of wide variety of microorganisms and for determining efficiency of sanitization of containers, equipment surfaces, water miscible cosmetics etc.
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Intended use
Recommended for determining efficiency of sanitization of containers, equipment surfaces, water miscible cosmetics etc.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Tryptone | 15.000 |
| Soya Peptone | 5.000 |
| Sodium chloride | 5.000 |
| Soya lecithin | 10.000 |
| Polysorbate 80 (Tween 80) | 40.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Soyabean casein digest agar w/ 4% Polysorbate 80 and 1% Soya Lecithin is a ready to use solid media in glass bottle. The medium is pre-sterilized, hence it does not need sterilization. Medium in the bottle can be melted either by using a pre-heated water bath or any other method. Slightly loosen the cap before melting. When complete melting of medium is observed dispense the medium in tubes as butts /slants or in plates as desired and allow to solidify. If on plate, either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically.
Principle And Interpretation
Soyabean casein digest agar with Lecithin and Polysorbate 80 is used in RODAC (Replicate Organism Detection and Counting) plates (1) for the detection and enumeration of microorganisms present on surfaces of sanitary importances (2,3). Tryptone and Soya peptone provide nitrogenous compounds and other nutrients essential for microbial replication. Lecithin and polysorbate 80 (Tween 80) are neutralizers reported to inactivate residual disinfectants from where the sample is collected (4). Lecithin neutralizes quaternary ammonium compounds and polysorbate 80 neutralizes phenolic disinfectants, hexachlorophene, formalin and with lecithin ethanol (5). Collection of samples from areas before and after the treatment with disinfectant evaluates cleaning procedures in environmental sanitation. The presence and number of microorganisms is determined by the appearance of colonies on the agar surface (6). After counting the colonies, carry out biochemical testing for identification.
Type of specimen
Swabs of containers, Equipment surfaces, Water miscible cosmetics etc.
Specimen Collection and Handling
For swabs of containers, equipment surfaces, water miscible cosmetics samples follow appropriate techniques for handling specimens as per established guidelines (1). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations:
- Biochemical characterization is necessary to be performed on colonies from pure cultures for further identification.
- This medium is general purpose medium and may not support the growth of fastidious organisms.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Soybean-Casein Digest Agar w/ 4% polysorbate 80 & 1% soya lecithin in glass bottle. (The solid medium may tend to have liquid over it)
Colour and Clarity of prepared medium
Light yellow coloured opaque medium
Quantity of medium
100ml of medium in glass bottle.
pH
7.10-7.50
Cultural Response
Growth Promotion was observed after an incubation at 30-35°C for 18-24 hours for bacteria and for fungus <=5 days.
Recovery rate
Recovery rate is considered 100% for bacterial growth on Blood Agar and fungal growth on Sabouraud Dextrose Agar.
| Organism | Inoculum (CFU) | Growth | Observed Lot value (CFU) | Recovery | Incubation temperature | Incubation period |
|---|---|---|---|---|---|---|
| Growth promoting | ||||||
| Bacillus subtilis subsp. spizizenii ATCC 6633 (00003*) | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Staphylococcus aureus subsp. aureus ATCC 6538 (00032*) | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Escherichia coli ATCC 8739 (00012*) | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Pseudomonas aeruginosa ATCC 9027 (00026*) | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Salmonella Abony NCTC 6017 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| $Kocuria rhizophila ATCC 9341 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Streptococcus pneumoniae ATCC 6305 | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Clostridium sporogenes ATCC 19404 (00008*) | 50-100 | luxuriant | 35-100 | >=70% | 30-35 °C | 18-24 hrs |
| Candida albicans ATCC 10231 (00054*) | 50-100 | luxuriant | 35-100 | >=70% | 20-25 °C | <=5 d |
| Candida albicans ATCC 2091 (00055*) | 50-100 | luxuriant | 35-100 | >=70% | 20-25 °C | <=5 d |
| Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | Good-luxuriant | 25-70 | 50-70% | 30-35 °C | <=5 d |
| Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | Luxuriant | 35-100 | >=70% | 20-25 °C | <=5 d |
| Candida albicans ATCC 10231 (00054*) | 50-100 | Good-luxuriant | 25-70 | 50-70% | 30-35 °C | <=5 d |
| Candida albicans ATCC 2091 (00055*) | 50-100 | Good-luxuriant | 25-70 | 50-70% | 30-35 °C | <=5 d |
Key: (*) Corresponding WDCM numbers, ($) Formerly known as Micrococcus luteus
Storage and Shelf Life
On receipt store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
| Product Name | Soyabean Casein Digest Agar w/ 1% Soya lecithin and 4% Polysorbate 80 |
|---|---|
| SKU | SM5531C |
| Customized Product Available | No |
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