Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Yeast extract | 3.000 |
| Gelatin Peptone | 7.000 |
| Bile salts | 1.500 |
| Sodium chloride | 5.000 |
| Glucose monohydrate | 10.000 |
| Neutral red | 0.030 |
| Crystal violet | 0.002 |
| Agar | 15.000 |
**Formula adjusted, standardized to suit performance parameters
Directions
Violet Red Bile Glucose Agar is a ready to use solid media in glass bottle. The medium is pre-sterilized; hence it does not need sterilization. Medium in the bottle can be melted either by using a pre-heated water bath or any other method. Slightly loosen the cap before melting. When complete melting of medium is observed dispense the medium as desired and allowed to solidify.
Principle And Interpretation
Violet Red Bile Glucose Agar is a selective medium recommended for detection and enumeration of Enterobacteriaceae especially the bile tolerant gram negative bacteria in accordance with the microbial limit testing by harmonized methodology of USP/EP/BP/JP/IP (1,2,3,4,5) from non-sterile products and pharmaceutical preparations.
Pancreatic digest of gelatin and yeast extract provide nitrogenous compounds and other nutrients essential for bacterial metabolism. This media is selective due to presence of the inhibitors; bile salts and crystal violet. Crystal violet inhibits gram-positive organisms especially Staphylococci. Neutral red indicator helps to detect glucose fermentation. Glucose fermenting strains produce red colonies with pink-red halos in the presence of neutral red. Sodium chloride maintains the osmotic equilibrium in the medium. The red colour is due to absorption of neutral red and a subsequent colour change of the dye when the pH of medium falls below 6.8.
Quality Control
Appearance: Sterile Violet Red Bile Glucose Agar in bottles.
Colour: Reddish purple coloured medium.
Quantity of Medium: 500 ml of medium in bottles.
pH of medium: 7.20-7.60
Growth Promotion Test
Growth Promotion is carried out in accordance with the harmonized method of USP/EP/BP/JP. Cultural response was observed after an incubation at 30-35°C for 18-24 hours. Recovery rate is considered as 100% for bacteria growth on Soyabean Casein Digest Agar.
Growth promoting properties
Growth of microorganism comparable to that previously obtained with previously tested and approved lot of medium occurs at the specified temperature for not more than the shortest period of time specified inoculating <=100 cfu (at 30-35° C for 18 hours).
Indicative properties
Colonies are comparable in appearance and indication reaction to those previously obtained with previously tested and approved lot of medium occurs for the specified temperature for a period of time within the range specified inoculating 100 cfu (at 30-35°C for 18-72 hours).
Sterility test
Passes release criteria.
Microbiological Test Results
Growth Promoting + Indicative
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of colony | Incubation temperature |
|---|---|---|---|---|---|
| Escherichia coli ATCC 8739 | 50-100 | luxuriant | >=50% | pink-red with bile precipitate | 18-24 hrs |
| Pseudomonas aeruginosa ATCC 9027 | 50-100 | luxuriant | >=50% | pink to red | 18-24 hrs |
Additional Microbiological Testing
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of colony | Incubation temperature |
|---|---|---|---|---|---|
| Escherichia coli NCTC 9002 | 50-100 | good-luxuriant | >=50% | pink-red with bile precipitate | 18-24 hrs |
| Escherichia coli ATCC 25922 | 50-100 | good-luxuriant | >=50% | light pink | 18-24 hrs |
| Salmonella Enteritidis ATCC 13076 | 50-100 | good-luxuriant | >=50% | pink-red | 18-24 hrs |
| Enterobacter aerogenes ATCC 13048 | 50-100 | good-luxuriant | >=50% | pink-red with bile precipitate | 18-24 hrs |
| Staphylococcus aureus ATCC 25923 | >=10³ | inhibited | 0% | - | >=24 hrs |
| Staphylococcus aureus ATCC 6538 | >=10³ | inhibited | 0% | - | >=24 hrs |
Storage and Shelf Life
Store between 15-25°C. Use before expiry date on the label.
| Product Name | Violet Red Bile Glucose Agar |
|---|---|
| SKU | SMH581D |
| Customized Product Available | No |
Write Your Own Review