Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended use
Recommended for detection of starch hydrolyzing microorganisms.
Composition**
| Ingredients | g/L |
|---|---|
| Peptone | 5.000 |
| Sodium chloride | 5.000 |
| Yeast extract | 1.500 |
| HM peptone B# | 1.500 |
| Starch, soluble | 2.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
# - Equivalent to Beef extract
Directions
Starch Agar is a ready to use solid media in glass bottle. The medium is pre-sterilized, hence it does not need sterilization. Medium in the bottle can be melted either by using a pre-heated water bath or any other method. Slightly loosen the cap before melting. When complete melting of medium is observed dispense the medium in tubes as butts /slants or in plates as desired and allow to solidify. If on plate, either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically.
Principle And Interpretation
Starch Agar was formulated by Vedder (1) in 1915, for the cultivation of Neisseria. Since then, other media have been developed that are superior to Starch Agar for the isolation of Neisseria species, including enriched GC Medium Base. Starch Agar (M107) is recommended for the detection of starch hydrolyzing microorganisms from foods (2) and clinical samples (3). Although the medium was originally formulated to perform the test for the identification of Bacillus cereus, it can be applied to any kind of microorganism where starch hydrolysis activity is required to be analyzed. Peptone, yeast extract and HM peptone B extract provide nitrogenous compounds, carbon, sulphur, trace elements etc. to the microorganisms. Sodium chloride maintains osmotic equilibrium. Flood the surface of 48 hours old culture on Starch Agar with Grams Iodine (S013). Starch hydrolysis is seen as a colourless zone surrounding the colonies. A blue or purple zone indicates that starch is not hydrolyzed. Size of the clear zone is directly proportional to the starch hydrolyzing activity of the strain under study.
Type of specimen
Isolated Microorganisms
Specimen Collection and Handling:
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (4,5). For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards. (6) After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations:
- Due to nutritional variations, some strains may show poor growth.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile glass bottle containing slightly opalescent Starch Agar
Colour of medium: Light amber coloured medium
Quantity of medium: 500 ml of medium in glass bottle.
pH: 7.20-7.60
Sterility Check: Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. ($ - on addition of Iodine solution)
| Organism | Inoculum (CFU) | Growth | Starch hydrolysis $ |
|---|---|---|---|
| ** Bacillus spizizenii ATCC 6633 (00003*) | 50-100 | luxuriant | positive reaction,clearing around the colony |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | negative reaction |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00032*) | 50-100 | luxuriant | negative reaction |
| Streptococcus pyogenes ATCC 19615 | 50-100 | luxuriant | negative reaction |
*- Corresponding WDCM numbers
**Formerly known as Bacillus subtilis subsp. spizizenii
Storage and Shelf Life
On receipt store between 15-30°C Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
| Product Name | Starch Agar |
|---|---|
| SKU | SM107D |
| Customized Product Available | No |
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