Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended use
Recommended for the isolation and enumeration of yeasts and moulds from water, dairy and other food products
Composition
| Ingredients | g/L |
|---|---|
| Infusion from potatoes from 200g | 4.000 |
| Dextrose (Glucose) | 20.000 |
| Agar | 15.000 |
| pH after sterilization (at 25°C) | 5.6±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Potato Dextrose Agar is a ready to use solid media in glass bottle. The medium is pre-sterilized, hence it does not need sterilization. Medium in the bottle can be melted either by using a pre-heated water bath or any other method. Slightly loosen the cap before melting. When complete melting of medium is observed dispense the medium in tubes as butts /slants or in plates as desired and allow to solidify. If on plate, either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically.
Principle And Interpretation
Potato Dextrose Agar is recommended by APHA (1) and F.D.A.(2) for plate counts of yeasts and moulds in the examination of foods and dairy products (3). Potato Dextrose Agar is also used for stimulating sporulation, for maintaining stock cultures of certain dermatophytes and for differentiation of typical varieties of dermatophytes on the basis of pigment production (4). It is also recommended by USP (5), BP (6), EP (3) and JP (7) for growth of fungi. Potato infusion and dextrose promote luxuriant fungal growth. Adjusting the pH of the medium by tartaric acid to 3.5, inhibits the bacterial growth. Heating the medium after acidification should be avoided as it may hydrolyse the agar which can render the agar unable to solidify.
Type of specimen
Food and dairy samples; Water samples
Specimen Collection and Handling
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (1,8). For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (9). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- For heavily contaminated samples, the media must be supplemented with inhibitory agents for inhibiting bacterial growth with lower pH.
- Some pathogenic fungi may produce infective spores which are easily dispersed in air, so examination should be carried out in safety cabinet.
- Further biochemical tests should be carried out for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile glass bottle containing slightly opalescent Potato Dextrose Agar
Colour of medium: Light amber coloured medium.
Quantity of medium: 100 ml of medium in glass bottle.
pH: 5.40-5.80
Sterility Check: Passes release criteria
Cultural Response: Cultural characteristics observed after an incubation at 22 - 25°C for 4 - 5 days
| Organism | Growth | Ascospore formation |
|---|---|---|
| # Aspergillus brasiliensis ATCC 16404 (00053*) | luxuriant | Negative |
| Candida albicans ATCC 10231 (00054*) | luxuriant | Negative |
| Saccharomyces cerevisiae ATCC 9763 (00058*) | luxuriant | Positive |
Key: (*) - corresponding WDCM numbers
(#) - Formerly known as Aspergillus niger
Storage and Shelf Life
On receipt store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (10,11).
| Product Name | Potato Dextrose Agar |
|---|---|
| SKU | SM096 |
| Customized Product Available | No |
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