Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended use
Recommended for the cultivation of less fastidious microorganisms, can be enriched with blood or other biological fluids.
Composition
| Ingredients | g/L |
|---|---|
| Peptone | 5.000 |
| Sodium chloride | 5.000 |
| HM peptone B# | 1.500 |
| Yeast extract | 1.500 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
# - Equivalent to Beef extract
Directions
Nutrient Agar is a ready to use solid media in glass bottle. The medium is pre-sterilized, hence it does not need sterilization. Medium in the bottle can be melted either by using a pre-heated water bath or any other method. Slightly loosen the cap before melting. When complete melting of medium is observed dispense the medium in tubes as butts /slants or in plates as desired and allow to solidify. If on plate, either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically.
Principle And Interpretation
Nutrient media are basic culture media used for maintaining microorganisms, cultivating fastidious organisms by enriching with serum or blood and are also used for purity checking prior to biochemical or serological testing (1,2). Nutrient Agar is ideal for demonstration and teaching purposes where a more prolonged survival of cultures at ambient temperature is often required without risk of overgrowth that can occur with more nutritious substrate. This relatively simple formula has been retained and is still widely used in the microbiological examination of variety of materials and is also recommended by standard methods. It is one of the several non-selective media useful in routine cultivation of microorganisms (3,4). It can be used for the cultivation and enumeration of bacteria which are not particularly fastidious. Addition of different biological fluids such as horse or sheep blood, serum, egg yolk etc. makes it suitable for the cultivation of related fastidious organisms. Peptone, HM peptone B and yeast extract provide the necessary nitrogen compounds, carbon, vitamins and also some trace ingredients necessary for the growth of bacteria. Sodium chloride maintains the osmotic equilibrium of the medium.
Type of specimen
Clinical samples - faeces, urine, etc; Food and dairy samples; Water samples
Specimen Collection and Handling:
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (3,4).
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (5).
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (6,7).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
In Vitro diagnostic Use. For professional use only. Read the label before opening the container. Wear protective gloves/ protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label expiry period when stored atwithin the recommended temperature.
Quality Control
Appearance
Sterile glass bottle containing slightly opalscent Nutrient Agar.
Colour of medium
Light yellow coloured medium
Quantity of medium
250ml of medium in glass bottle.
pH
7.20-7.60
Sterility Check
Passes release criteria
Cultural Response
Productivity: Cultural characteristics observed after melting the medium and pouring into sterile Petri plates. The plates are inoculated with following test organisms and incubation at 35-37°C for 18-48 hours. Recovery rate is considered as 100% for bacteria growth on Soyabean Casein Digest Agar.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Productivity | |||
| Escherichia coli ATCC 25922 (00013*) | 50-100 | good-luxuriant | >=70% |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | good-luxuriant | >=70% |
| Salmonella Typhi ATCC 6539 | 50-100 | good-luxuriant | >=70% |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | good-luxuriant | >=70% |
| Streptococcus pyogenes ATCC 19615 | 50-100 | good-luxuriant | >=70% |
| Salmonella Enteritidis ATCC 13076 (00030*) | 50-100 | good-luxuriant | >=70% |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | good-luxuriant | >=70% |
| Yersinia enterocolitica ATCC 9610 (00038*) | 50-100 | good-luxuriant | >=70% |
| Yersinia enterocolitica ATCC 23715 (00160*) | 50-100 | good-luxuriant | >=70% |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (6,7).
| Product Name | Nutrient Agar |
|---|---|
| SKU | SM001CCL |
| Customized Product Available | No |
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