Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
For cultivation of Mycobacterium tuberculosis.
Composition**
| Ingredients | Gms / 600ml |
|---|---|
| L-Asparagine | 3.600 |
| Monopotassium phosphate | 2.400 |
| Magnesium sulphate | 0.240 |
| Magnesium citrate | 0.600 |
| Potato starch, soluble | 30.000 |
| Malachite green | 0.400 |
| Glycerol | 12.000 ml |
| Whole Egg Emulsion | 1000.000 ml |
| p-Aminosalicylic acid | 1.00µg/ml |
**Formula adjusted, standardized to suit performance parameters
Directions
Inoculate either the sputum sample previously subjected to decontamination and concentration process or the pure culture of Mycobacteria isolated from a clinical sample on the surface of the slants. Incubate the slants at 35-37°C with 5-10% CO2 and examine the slants every week up to 8 weeks.
Principle And Interpretation
L.J. Medium is prepared as per the Jensen's (1) modification of the original formulation of Lowenstein (2). The egg base medium supports a wide variety of Mycobacteria and can also be used for niacin testing (3). Glycerol provides fatty acids. Malachite green serves as an inhibitor as well as pH indicator. Formation of blue zones indicates a decrease in pH by Gram-positive contaminants (e.g. Streptoccocci ) and yellow zones of dye destruction by Gram-negative bacilli. Proteolytic contaminants cause localized or complete digestion of the medium (4).
Quality Control
Appearance
Pale bluish green coloured, opaque smooth slant containingp-Aminosalicylic acid (1 µg/ml) in long tube(165mmX16mm).
Sterility test
Passes release
Cultural response
Cultural characteri tics within 10 to 15 days at 35°C, with 5-10% CO2(further growth may be observed for 2 to 4 weeks)
| Organism | Inoculum | Growth on control | Growth on slant w/ PASA | Colony characteristics |
|---|---|---|---|---|
| M. tuberculosis H37Rv ATCC 25618 | Standardized inoculum giving approximately 1000000 cfu/ml | Luxuriant | None to poor | Granular, rough, dry warty, friable colonies |
Storage and Shelf Life
On reciept Store between 2-8°C. Use before expiry date on the label.
| Product Name | L.J Medium Slant w/ p-Amino salicylic acid (1µg/ml) (long tube) |
|---|---|
| SKU | SL171L |
| Customized Product Available | No |
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