Tuberculosis Second Line Kit (Total 10 slants)

Principle And Interpretation

Based on invitro correlation between the clinical response to antimicrobial agent and the result of invitro susceptibility testing kit (SL023) helps in diagnosing the sensitivity pattern of M. tuberculosis affected patient and accordingly provide treatment, drug therapy for the patients.

Primary drug resistance for M. tuberculosis is defined as resistance to an antimicrobial drug in an organism isolated from a patient who has not previously received antituberculosis therapy. Therefore drug resistance applies to previously untreated patients who are found to have drug resistant organisms presumably because they have been infects from an outside source of resistant M. tuberculosis. Acquired (or secondary) drug resistance is due to importantly because of non-adherence in drug taking.

Mycobacteria susceptibility test can be inoculated either directly from digested and concentrated smear positive sputum (direct test) or from a pure culture of Mycobacteria isolated from a clinical specimen (indirect test). The direct test is usually done only on specimens showing Mycobacteria on smear and give the best results when large no. of Mycobacteria are present. The advantage of the direct test is that a much earlier report of susceptibility studies (3 to 4 weeks) can be made than with indirect test which may take up to 5 to 7 weeks, but can be frequently be complicated by over growth with other bacteria that have survived the decontamination procedure.