Kit contains five anti-tubercular agents (Isoniazide, Streptomycin, Ethambutol, Rifampicin & Pyrazinamide) + 2 control . This medium is recommended for cultivation of Mycobacterium tuberculosis and for resistance testing. Lowenstein Jensen Medium Base w/o Starch is recommended for drug resistance testing of Mycobacteria as recommended by WHO. Penicillin and nalidixic acid (FD053) along with malachite green prevents growth of the majority of contaminants . RNA (in FD053) acts as stimulant and helps to increase the isolation rate of Mycobacteria.