Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for cultivation of Corynebacteria, especially Corynebacterium diphtheriae.
Composition**
| Ingredients | g / 250ml |
|---|---|
| Peptone, special | 2.500 |
| HM peptone B # | 2.500 |
| Sodium chloride | 1.250 |
| Dextrose (Glucose) | 2.500 |
| Horse serum | 750.00 ml |
| Final pH (at 25°C) | 7.3±0.2 |
# Equivalent to Beef extract
**Formula adjusted, standardized to suit performance parameters
Directions
Streak the test inoculum aseptically into the slant and incubate at appropriate conditions. Incubate the slants at 30-35°C for 18-24 hours.
Principle And Interpretation
Loeffler Medium was originally devised by Loeffler (1) and was further modified by Perry and Petran (2) and Buck (3). Loeffler medium enhances primary and secondary isolation and cultivation of fastidious pathogenic microorganisms especially from nose and throat. It also restores virulence and other identifying properties (microscopic and colonial) after they have been lost due to prolonged incubation or repeated subculturing.
The high serum content helps in determining proteolytic activity of organisms. It is also used for demonstration of pigmentation and ascospores. Peptone special, HM peptone B provide essential growth nutrients. Dextrose is the source of fermentable carbohydrate and energy.
Rub the swabs directly over the surface of medium and after incubation; prepare the smears from surface of slope. For proteolysis testing, inoculate slant and prior to incubation, flood the slant with Brewer Thioglycollate Medium (M019). Incubation should be carried out for 3-4 days or much longer for appearance of proteolysis. Loeffler Medium should be used in parallel with Serum Tellurite Agar for selective isolation of Corynebacteria (1).
Type of specimen
Clinical samples- a nasopharyngeal and throat swabs
Specimen Collection and Handling:
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (4,5). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions:
In Vitro diagnostic use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- For optimum recovery of C. diphtheriae, a nasopharyngeal and throat swab should be obtained while collection of specimen.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Loeffler's Medium slant in glass tube.
Colour of medium
Yellowish white coloured medium
Quantity of Medium
8ml of medium in glass tube
Reaction
7.10-7.50
Sterility Test
Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Corynebacterium diphtheriae ATCC 11913 | 50-100 | Good |
| Corynebacterium diphtheriae type mitis ATCC 8026 | 50-100 | Good |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | L.M. Slant (Loeffler Medium) |
|---|---|
| SKU | SL019 |
| Customized Product Available | No |
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