Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use:
Recommended for cultivation of Mycobacterium tuberculosis.
Composition**
| Ingredients | g/600 ml |
|---|---|
| L-Asparagine | 3.60 |
| Potassium dihydrogen phosphate | 2.40 |
| Magnesium sulphate | 0.24 |
| Magnesium citrate | 0.60 |
| Potato starch, soluble | 30.00 |
| Malachite green | 0.40 |
| Glycerol | 12.00 ml |
| Whole Egg Emulsion | 1000.00 ml |
**Formula adjusted, standardized to suit performance parameters
Directions
Follow aseptic techniques. Inoculate either the sputum sample previously subjected to decontamination and concentration process or the pure culture of Mycobacteria isolated from a clinical sample on the surface of the slants. Incubate the slants at 30-35°C with 5-10% CO2 and examine the slants every week up to 8 weeks.
Principle And Interpretation
L.J. Medium is prepared as per the Jensen's (1) modification of the original formulation of Lowenstein (2). The egg base medium supports a wide variety of Mycobacteria and can also be used for niacin testing (3). Glycerol provides fatty acids. Malachite green serves as an inhibitor as well as pH indicator. Formation of blue zones indicates a decrease in pH by Gram-positive contaminants (e.g. Streptoccocci) and yellow zones of dye destruction by Gram-negative bacilli. Proteolytic contaminants cause localized or complete digestion of the medium (4). This medium supports the growth of a wide variety of Mycobacteria and can also be used for niacin testing (3). Hardy et al (5) recommended each specimen to be inoculated and incubated in triplicate.
Type of specimen
Isolated organism and Clinical specimens such as sputum
Specimen Collection and Handling
For clinical samples: Refer appropriate references for standard test procedures of decontamination and isolation (6,7,8,9). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Certain gram positive contaminants (e.g. Streptococci) and gram-negative bacilli may grow on the medium.
- 2. Certain Saprophytes may also grow on the medium.
- 3. Proteolytic contaminants cause localized or complete digestion of medium.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance:
Sterile Lowenstein Jensen Medium: pale bluish green coloured, opaque, smooth slants in long tube.
Sterility test:
Passes release criteria
Cultural response:
Cultural characteristics within 2-4 weeks at 35-37°C, with 5-10% CO2 (further growth may be observed for 8 weeks)
| Organism | Inoculum | Growth | Colony characteristics |
|---|---|---|---|
| Mycobacterium gordonae ATCC 14470 | Standardized inoculum giving approximately 1000000 cfu/ml | Luxuriant | Smooth, yellowish orange colonies. |
| Mycobacterium kansasii ATCC 14278 | Standardized inoculum giving approximately 1000000 cfu/ml | Luxuriant | Smooth-rough, photochromogenic colonies. |
| Mycobacterium smegmatis ATCC 14468 | Standardized inoculum giving approximately 1000000 cfu/ml | Luxuriant | Wrinkled, creamy white colonies. |
| M. tuberculosis H37Rv ATCC 25618 | Standardized inoculum giving approximately 1000000 cfu/ml | Luxuriant | Granular, rough, warty, dry, friable colonies. |
| M. avium subsp. Avium MTCC 1723 | Standardized inoculum giving approximately 1000000 cfu/ ml | Luxuriant | Smooth, nonpigmented colonies. |
Storage and Shelf Life
On receipt store between 2-8°C.Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (6,7,8,9).
| Product Name | L.J. Medium Slant (Long tube) |
|---|---|
| SKU | SL001L |
| Customized Product Available | No |
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