Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Sabouraud Dextrose Agar w/ 0.5% Polysorbate 80 & 0.07% Soya lecithin
Recommended for determining efficiency of sanitization of containers, equipment surfaces, water miscible cosmetics etc.
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Intended use
Recommended for the cultivation of yeasts, moulds and aciduric bacteria and can be used for determining efficiency of sanitization of containers, equipment surfaces, water miscible cosmetics etc.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Mycological peptone | 10.000 |
| Dextrose(Glucose) | 40.000 |
| Polysorbate 80 (Tween 80) | 5.000 |
| Soya lecithin | 0.700 |
| Agar | 15.000 |
| Final pH (at 25°C) | 5.6±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Sabouraud Dextrose Agar w/0.5% Polysorbate 80 & 0.07% Soya lecithin is a ready to use solid media in glass bottle. The medium is pre-sterilized, hence it does not need sterilization. Medium in the bottle can be melted either by using a pre-heated water bath or any other method. Slightly loosen the cap before melting. When complete melting of medium is observed dispense the medium in tubes as butts /slants or in plates as desired and allow to solidify. If on plate, either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically.
Caution: Some pathogenic fungi may produce infective spores which are easily dispersed in air, so examination should be carried out in safety cabinet.
Principle And Interpretation
Sabouraud Dextrose Agar with Soya Lecithin and Polysorbate 80 is the modification of formulation described by Sabouraud for determining efficiency of sterilization of container etc. with respect to yeast moulds and aciduric bacteria. Mycological peptone provides nitrogenous compounds. Dextrose provides an energy source. The low pH favours fungal growth and inhibits contaminating bacteria from clinical specimens (1). Some pathogenic fungi may produce infective spores which are easily dispersed in air, so examination should be carried out in safety cabinet. Lecithin and polysorbate 80 are neutralizers reported to inactivate residual disinfectants from where the sample is collected (2). Lecithin neutralizes quartenery ammonium compounds and polysorbate 80 neutralizes phenolic disinfectants, hexachlorophene formalin, and with lecithin neutralizes ethanol (3). Collection of samples from areas before and after the treatment with disinfectant evaluates cleaning procedures in environmental sanitation. The presence and number of fungi is determined by the appearance of colonies on the agar surface.
Type of specimen
Environmental samples
Specimen Collection and Handling
For environmental samples, follow appropriate techniques for sample collection and processing as per guidelines (4,5). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- 1. Some pathogenic fungi may produce infective spores which are easily dispersed in air, so examination should be carried out in safety cabinet.
- 2. Further serological or biochemical testing is required for complete identification.
- 3. Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance Sterile glass bottle containing clear to slightly opalescent Sabouraud Dextrose Agar w/0.5% Polysorbate 80 & 0.07% Soya lecithin
Colour of medium Light amber coloured medium
Quantity of medium 100 ml of medium in glass bottle.
Reaction 5.40-5.80
Sterility Check Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 20-25°C for 48-72 hours (Incubate for 7 days for Trichophyton species).
| Organism | Inoculum (CFU) | Growth | Recovery | Incubation temperature | Incubation period |
|---|---|---|---|---|---|
| #Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | Luxuriant (white colonies) | ≥70% | 25°C | 3Days |
| Candida albicans ATCC 2091 (00055*) | 50-100 | luxuriant | ≥70% | 25°C | 48Hours |
| Candida albicans ATCC 10231 (00054*) | 50-100 | luxuriant | ≥70% | 25°C | 48Hours |
| Trichophyton rubrum ATCC 28191 | - | luxuriant | - | 25°C | 5Days |
| Saccharomyces cerevisiae ATCC 9763 (00058*) | 50-100 | luxuriant | ≥70% | 25°C | 48Hours |
Key: (*) - Corresponding WDCM numbers, (#) - Formerly known as Aspergillus niger
Storage and Shelf Life
On receipt store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | Sabouraud Dextrose Agar w/ 0.5% Polysorbate 80 & 0.07% Soya lecithin |
|---|---|
| SKU | SM5526C |
| Customized Product Available | No |
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