Haematoxylin and Eosin is the most commonly used stain, which is specific for certain substances of diagnostic importance. Here, acid reacting components of the cell combine with alkaline dyes and the alkaline area react with acid dyes. This stain is available for amyloid, lipids, inorganic substances such as iron and calcium, pigments like melanin and hemosiderin, carbohydrates and mucopolysaccharides.