Folin & Ciocalteu′s phenol reagent is most commonly used in the Lowry method for determining protein concentration.It has also been used for the quantification of total phenolics.
Folin & ciocalteu’s phenol reagent,also called the gallic acid equivalence method (GAE), is a mixture of phosphomolybdate and phosphotungstate used for the colorimetric in vitro assay of phenolic and polyphenolic antioxidants.