Benedict's reagent is used to test for the presence of reducing sugars.Benedict's reagent is used to test for the presence of glucose in urine. Once a reducing sugar is detected in urine,further tests have to be undergone in order to ascertain which sugar is present. The original colour of Benedict's reagent is blue. It changes to green, yellow, orange or red, according to the concentration of glucose present in urine.Benedict’s Qualitative Reagent use for detection of sugar in Urine.