Tomato Regeneration Medium has been formulated on the basis of Murashige and Skoog medium for the in vitro regeneration of Solanum lycopersicum, family Solanaceae. It is widely used for the micro propagation,protoplast culture, callus culture and suspension culture of tomato. The formulation is a nutrient blend of inorganic salts, vitamins, amino acid, carbohydrate,growth regulator and gelling agent.