Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Mehlman's Maintenance HiVeg Medium is recommended for maintenance of Campylobacter species.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone No. 3 | 15.0 |
| Yeast extract | 7.5 |
| HiVeg hydrolysate | 5.0 |
| Dipotassium phosphate | 5.0 |
| Starch, soluble | 1.0 |
| Ammonium sulphate | 1.5 |
| Neutral red | 0.02 |
| Agar | 3.0 |
Final pH (at 25°C) 7.3 ± 0.2
Formula adjusted, standardized to suit performance parameters.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) | ||
|---|---|---|---|
| MV917 | HiVeg peptone No. 3 HiVeg hydrolysate |
M917 | Proteose peptone Casein enzymic hydrolysate |
| Recommended for | Maintenance of Campylobacter species. |
| Reconstitution | 38.0 g/l |
| Quantity on preparation (500g) | 13.15 L |
| pH (25°C) | 7.3 ± 0.2 |
| Supplement | Sodium sulphite |
| Sterilization | 121°C / 15 minutes |
Storage and Shelf Life
Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Directions
Suspend 38 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense in 8 ml amounts in screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45 - 50°C and aseptically add 0.2 ml filter sterilized 2.5% sodium sulphite solution to each tube. Mix gently.
Principle and Interpretation
Mehlman's Maintenance HiVeg Medium is prepared by using HiVeg peptone No.3 and HiVeg hydrolysate which are free from BSE/TSE risks associated with animal based peptones. Mehlman's Maintenance HiVeg Medium is the modification of Mehlman's Maintenance Medium which is recommended by APHA (1) for the maintenance of Campylobacter species. Organisms of the genus Campylobacter were originally classified in the genus Vibrio. Most strains now associated with acute gastroenteritis in humans and their ability to grow at 42°C were originally described as related Vibrios (2).
The medium contains nitrogenous nutrients such as HiVeg peptone No.3, yeast extract and HiVeg hydrolysate. The medium also contains starch but lack the energy source such as dextrose. Neutral red is the pH indicator. Dipotassium phosphate helps in maintaining buffering action of the medium.
Quality Control
Appearance of powder
Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Semisolid, comparable with 0.3% Agar gel.
Colour and Clarity
Orange red coloured, clear to slightly opalescent solution.
Reaction
Reaction of 3.8% w/v aqueous solution is pH 7.3 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C upto 1 week.
| Organisms (ATCC) | Growth |
|---|---|
| Campylobacter coli (33559) | good-luxuriant |
| Campylobacter jejuni (29428) | good-luxuriant |
References
- Speck (Eds.), 1984, Compendium of Methods For The Microbiological Examination of Foods, 2nd ed., APHA, Washington, D.C.
- King E.O., 1957, J. Infect. Dis., 101: 119.
| Product Name | Mehlman’s Maintenance HiVeg™ Medium |
|---|---|
| SKU | MV917 |
| Product Type | HiVegâ„¢ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. King E. O., 1957, J. Infect. Dis., 101: 11 |
| Customized Product Available | No |
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