Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for selective isolation and biochemical differentiation of Yersinia enterocolitica.
Composition**
| Ingredients | g/L |
|---|---|
| Yeast extract | 3.000 |
| Sodium chloride | 5.000 |
| Cellobiose | 3.500 |
| L-Arginine | 6.500 |
| L-Lysine hydrochloride | 6.500 |
| Synthetic detergent No. III | 1.500 |
| Neutral red | 0.030 |
| Agar | 20.000 |
| Final pH (at 25°C) | 7.1±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 46.03 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. DO NOT OVERHEAT OR AUTOCLAVE. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Yersinia enterocolitica is a significant invasive enteric pathogen belonging to the family Enterobacteriaceae, which causes several well-recognized diseases especially in younger persons and several uncommon post-infection syndromes (1). Enterocolitis caused by Y. enterocolitica is characterized by diarrhoea, low fever and abdominal pain. CAL Agar used for selective isolation of Y. enterocolitica was originally formulated by Dudley and Shotts (2). CAL Agar is a differential medium as it differentiate Yersinia on the basis of cellobiose fermentation and lysine or arginine decarboxylation. CAL Agar is generally used for the isolation and characterization of Y. enterocolitica from faecal specimens (2) as the organism is biochemically similar to other Enterobacteriaceae (3). CAL Broth is used for the enumeration of Y. enterocolitica from water and other liquid specimens (3). CAL HiVeg™™ Agar is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks.
Yeast extract provides essential nutrients to the organisms. Cellobiose is the fermentable carbohydrate. Sodium chloride maintains the osmotic equilibrium. Synthetic detergent No. III makes the medium selective by inhibiting the accompanying gram-positive bacteria, which may cause contamination during cultivation. L-arginine and L-lysine are the amino acids, decarboxylation of which makes the medium differential. Neutral red is the indicator, which turns red under acidic conditions.
Type of specimen
Food and dairy samples
Specimen Collection and Handling:
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (4,5,6). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Further biochemical and serological tests must be carried out for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Light yellow to pink homogeneous free flowing powder
Gelling
Firm, comparable with 2.0% Agar gel.
Colour and Clarity of prepared medium
Red coloured, clear to slightly opalescent gel forms in Petri plates.
Reaction
Reaction of 4.6% w/v aqueous solution at 25°C. pH: 7.1±0.2
pH
6.90-7.30
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth | Cellobiose | Arginine Decarboxylation | Lysine Decarboxylation |
|---|---|---|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | 50-100 | good | negative reaction | variable reaction | variable reaction |
| Proteus mirabilis ATCC 25933 | 50-100 | good | negative reaction | negative reaction | negative reaction |
| Pseudomonas aeruginosa ATCC 27853(00025*) | 50-100 | good | negative reaction | negative reaction | positive reaction |
| Yersinia enterocolitica ATCC 27729 | 50-100 | good-luxuriant | positive reaction | negative reaction | negative reaction |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
| Product Name | Cellobiose Arginine Lysine HiVeg™ Agar (CAL HiVeg™ Agar) |
|---|---|
| SKU | MV893 |
| Customized Product Available | No |
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