Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for cultivation of organisms from body fluids and other materials containing Penicillin, Streptomycin and Sulphonamides.
Composition**
| Ingredients | g/L |
|---|---|
| HiVeg™ peptone No. 3 | 10.000 |
| Yeast extract | 5.000 |
| Dextrose (Glucose) | 1.000 |
| Sodium chloride | 5.000 |
| Thiol compound | 8.000 |
| p-Amino benzoic acid (PABA) | 0.050 |
| Agar | 1.000 |
| Final pH (at 25°C) | 7.1±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 30.05 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Dispense in tubes or flasks to a depth of 6 cm for neutralization of Penicillin or in shallow layers for neutralization of Streptomycin. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Use within 4 days of preparation.
Principle And Interpretation
Thiol Medium is used for culturing microorganisms from body fluids and also other materials containing antibiotics like penicillin, streptomycin or sulphonamides. The efficacy of Thiol Medium to retain viability of Vibrio was initially described by Huddleson (1). The ability of Thiol Medium to neutralize antibacterials was demonstrated by Christensen (1). This media can also be used for the cultivation and maintenance of Haemophilus, Vibrio and Meningococci (2). Thiol HiVeg™ Medium is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks. HiVeg™ peptone No. 3 and yeast extract provide nitrogenous compounds, vitamin B complex and other essential growth nutrients. Dextrose is the energy source. The small quantity of agar keeps the oxido-reductive potential quite congenial for the growth of aerobic, microaerophilic and anaerobic microorganisms. p-Amino benzoic acid serves as a preservative.
Type of specimen
Please add specimens
Specimen Collection and Handling:
10 ml of Thiol Medium has capacity to nullify 100 units of penicillin and 1000 units of streptomycin supporting good growth of Staphylococci and other test organisms. Even dilute inocula of the test organisms can initiate and result in good growth within 24 hours. For testing, medium is prepared and tested with and without concentrations of 5, 100 and 1000 units of penicillin and 100, 1000 and 10,000 micrograms of streptomycin per 10 ml of tube. It is further inoculated with test organisms and incubated at 18 - 48 hours at 35-37°C After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- 1. Use the medium within 4 days of preparation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Viscous, comparable with 0.1% Agar gel.
Colour and Clarity of prepared medium
Light yellow coloured clear to slightly opalescent solution.
Reaction
Reaction of 3.0% w/v aqueous solution at 25°C. pH: 7.1±0.2 pH
6.90-7.30
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours. Growth observed after addition of antibiotic concentrations upto 100 units of Penicillin or 1,000 micrograms of Streptomycin.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Neisseria meningitidis ATCC 13090 | 50-100 | poor-fair |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | good-luxuriant |
| Streptococcus pneumoniae ATCC 6303 | 50-100 | good-luxuriant |
| Streptococcus pyogenes ATCC 19615 | 50-100 | good-luxuriant |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Thiol HiVeg™ Medium |
|---|---|
| SKU | MV852 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Huddleson I. F., 1948, J. Bacteriol., 56:508. |
| Customized Product Available | No |
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Thiol Medium
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