M-Broth, HiVeg™

Principle and Interpretation

M-Broth, HiVeg is prepared by using vegetable peptones in place of animal based peptones which makes the medium free of BSE/TSE risks.

M-Broth, HiVeg is the modification of M-Broth which was developed by Sperber and Diebel (1) to accelerate the detection of Salmonellae.

The accelerated 50 hour detection procedure consists of 18 hours pre-enrichment, 24 hours selective enrichment, 6 to 8 hours selective enrichment and 2 hours serological testing.

This medium contains D-Mannose for the selective enrichment step to avoid nonspecific agglutination, that can occur with dextrose.

Fantasia, Sperber and Deibel (2) found that enrichment serology method is rapid and less complicated to perform than the method described in the Bacteriological Analytical Manual (3) by maintaining the accuracy and sensitivity of the method.

HiVeg hydrolysate, yeast extract provides organic nitrogen, carbon, sulphur, vitamins and trace elements essential for the growth of Salmonella species.

Mannose is the fermentable sugar and energy source and it prevents fimbrial agglutination of Salmonella (1).

The inorganic salts stimulate bacterial growth while polysorbate 80 supplies fatty acids.

10% suspension of sample is prepared in sterile Fluid Lactose HiVeg Broth (MV026) and incubated at 35 ± 2°C for 18 - 24 hours. 1 ml of this pre-enriched culture is added to 9 ml of Selenite Cystine HiVeg Broth Base (MV025) and Tetrathionate HiVeg Broth Base (MV032) and incubated at 35 ± 2°C for 24 hours. This enriched culture is inoculated in M-Broth HiVeg and incubated at 35 ± 2°C for 6 hours and then H-agglutination test is performed.