Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use:
Recommended for studying fermentation by Staphylococcus and Micrococcus species.
Composition**
| Ingredients | g/L |
|---|---|
| HiVeg™ hydrolysate | 10.000 |
| Yeast extract | 1.000 |
| Dextrose (Glucose) | 10.000 |
| Bromo cresol purple | 0.040 |
| Agar | 2.200 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 23.24 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow the tubed medium to cool in an upright position.
Principle And Interpretation
Several methods are available for differentiating Micrococcus and Staphylococcus species. These two are the most frequently encountered catalase-positive genera in the clinical laboratory. Staphylococcus aureus is a primary pathogen, which may be associated with severe infection. Micrococci are gram-positive organisms that are generally strict aerobes and can reduce nitrate. Micrococcus luteus oxidizes carbohydrates to CO2 and water, and it does not produce acid from glucose anaerobically as well as it does not synthesize or possess arginine dihydrolase or β-galactosidase. The defining characteristics of Micrococcus are its ability to aerobically produce acid from glucose, esculin hydrolysis, major pigment production, motility, and conversion of nitrate to nitrite (1). Fermentation Medium for Staphylococcus and Micrococcus is recommended for differentiation of these two organisms on the basis of fermentation reaction. Staphylococcus produces acid from glucose anaerobically whereas Micrococcus fails to do so (2). This test is performed in a manner similar to the oxidation fermentation tests for non-fermentative organisms. Fermentation HiVeg™ Medium for Staphylococcus and Micrococcus is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/ TSE risks.
HiVeg™ hydrolysate and yeast extract provide necessary nitrogenous nutrients for the organisms. Glucose is the fermentable carbohydrate source in the medium. Bromo cresol purple is the pH indicator. Incorporation of small amount of agar in this medium helps to create anaerobic condition in the depths of the tubes.
Type of specimen
Food samples; Water samples
Specimen Collection and Handling:
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (3). For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(4) After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Further confirmed by gram staining and oxidase test.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Light yellow to greenish yellow homogeneous free flowing powder
Gelling
Semisolid, comparable with 0.22% Agar gel.
Colour and Clarity of prepared medium
Purple coloured, clear to slightly opalescent gel forms in tubes as butts
Reaction
Reaction of 2.32% w/v aqueous solution at 25°C. pH: 7.0±0.2
pH
6.80-7.20
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) |
Growth | Acid production |
|---|---|---|---|
| Micrococcus luteus ATCC 10240 | 50-100 | good-luxuriant | negative reaction, no colour change |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | good-luxuriant | positive reaction, yellow colour |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Fermentation HiVeg™ Medium for Staphylococcus and Micrococcus |
|---|---|
| SKU | MV827 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Smith K. J., Neafie R., Yeager J., and Skelton H. G., 1999, British Journal of Dermatology, Vol. 141, No. 3, BritishAssociation of Dermatologists, (558-561). |
| Customized Product Available | No |
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Fermentation Medium For Staphylococcus and Micrococcus, w/ 0.2% Agar
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