Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Tryptone Glucose Beef Extract HiVeg™ Agar (Revised as Tryptone Glucose HM Peptone B HiVeg ™ Agar)
Used for enumeration of bacteria in water, air, milk and other dairy products.
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Intended Use
TGB HiVeg Agar is recommended for enumeration of bacteria in water, air, milk and other dairy products.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV791 | M791 |
| HiVeg hydrolysate | Casein enzymic hydrolysate |
| HiVeg extract | Beef extract |
Recommended for
Enumeration of bacteria in water, air, milk and other dairy products.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate | 5.0 |
| HiVeg extract | 3.0 |
| Glucose | 1.0 |
| Agar | 15.0 |
Final pH (at 25°C) 7.0 ± 0.2
Formula adjusted, standardized to suit performance parameters.
Reconstitution
24.0 g/l
Quantity on Preparation
| (500g) | 20.83 L |
| (100g) | 4.16 L |
pH (25°C)
7.0 ± 0.2
Supplement
None
Sterilization
121°C / 15 minutes.
Storage
Dry Medium Below 30°C, Prepared Medium 2–8°C.
Directions
Suspend 24 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
TGB HiVeg Agar is prepared by replacing Casein enzymic hydrolysate and Beef extract of bovine origin by HiVeg hydrolysate and HiVeg extract respectively which are of vegetable origin, hence free of BSE/TSE risks. TGB HiVeg Agar is a modification of Glucose Skim Milk Agar designed by Bowers and Huker (1). It is used for cultivation and enumeration of bacteria in milk and other dairy products (2) and for the standard plate count of food (3) and water (4). HiVeg hydrolysate, HiVeg extract provide nitrogenous and carbonaceous compounds and other nutrients essential for the growth of organisms. Glucose serves as an energy source.
This medium can be also used for pour plate technique. Usually 1 ml samples of appropriate dilutions of the test sample are pipetted in to sterile petriplates and molten, cooled TGB HiVeg Agar is added followed by gentle mixing so as to get uniform mixing. Incubate plates for 48-72 hours at 35°C.
Quality Control
Appearance of powder: Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity: Light yellow coloured, clear to slightly opalescent gel forms in petri plates.
Reaction: Reaction of 2.4% w/v aqueous solution is pH 7.0 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Bacillus subtilis (6633) | 102-103 | luxuriant | >70% |
| Enterococcus faecalis (29212) | 102-103 | luxuriant | >70% |
| Escherichia coli (25922) | 102-103 | luxuriant | >70% |
| Lactobacillus casei (9595) | 102-103 | luxuriant | >70% |
| Pseudomonas aeruginosa (27853) | 102-103 | luxuriant | >70% |
| Staphylococcus aureus (25923) | 102-103 | luxuriant | >70% |
| Streptococcus pyogenes (19615) | 102-103 | luxuriant | >70% |
| Product Name | Tryptone Glucose Beef Extract HiVeg™ Agar (Revised as Tryptone Glucose HM Peptone B HiVeg ™ Agar) |
|---|---|
| SKU | MV791 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Bowers L. S. and Huker J. G., 1935, Tech. Bull. 228, N.Y. State Agr. Exp. Sta. |
| Customized Product Available | No |
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