Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Photobacterium HiVeg Broth is used for cultivation and demonstration of luminescence of photobacteria.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg hydrolysate | 5.0 |
| Yeast extract | 2.5 |
| Sodium chloride | 30.0 |
| Ammonium chloride | 0.3 |
| Magnesium sulphate | 0.3 |
| Ferric chloride | 0.01 |
| Calcium carbonate | 1.0 |
| Monopotassium dihydrogen phosphate | 3.0 |
| Sodium glycerophosphate | 23.5 |
Final pH (at 25°C) 7.0 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 65.61 grams in 1000 ml distilled water. Heat just to boiling. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Before pouring mix well to evenly distribute slight precipitate formed.
NOTE: Due to presence of calcium carbonate the prepared medium forms opalescent solution with white precipitate
Principle and Interpretation
Photobacterium HiVeg Broth is prepared by using HiVeg hydrolysate which is free of BSE/TSE risks. Photobacterium HiVeg Broth is the modification of Photobacterium Broth which is prepared based on the formulations described by Daudoroff (1) and Giese (2) for the cultivation and demonstration of luminescence of photobacteria.
HiVeg hydrolysate and yeast extract provide nitrogenous compounds, carbon, sulphur, trace nutrients, vitamin B complex, which is essential for the growth of photobacteria. Potassium dihydrogen phosphate helps in maintaining the buffering capacity of the medium. Chlorides, sulphate, carbonate and also the glycerophosphate helps in luminescence. The intensity of luminescence is related to the aeration of culture. The greater the oxygen supply, the greater will be luminescence.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV783 | M783 |
| HiVeg hydrolysate | Casein enzymic hydrolysate |
| Recommended for | : Cultivation and demonstration of luminescence of photobacteria. |
| Reconstitution | : 65.61 g/l |
| Quantity on preparation (100g) | : 1.52 L |
| pH (25°C) | : 7.0 ± 0.2 |
| Supplement | : None |
| Sterilization | : 121°C / 15 minutes. |
| Storage | : Dry Medium - Below 30°C, Prepared Medium 2 - 8°C. |
Quality Control
Appearance of powder
Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Colour and Clarity
Light amber coloured clear to slightly opalescent solution with heavy white precipitate.
Reaction
Reaction of 6.56% w/v aqueous solution is pH 7.0 ± 0.2 at 25°C
Cultural Response
Cultural characteristics observed after an incubation at 25 - 30°C for 18 - 24 hours.
Organisms (ATCC)
| Organisms (ATCC) | Growth | Luminescence |
|---|---|---|
| Lucibacterium harveyi (14126) | good-luxuriant | + |
| Vibrio fischeri (7744) | good-luxuriant | + |
| Product Name | Photobacterium HiVeg™ Broth |
|---|---|
| SKU | MV783 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Norton C.F., 1986, Microbiology, 2nd Ed., Addison-Wesley Publishing Company. |
| Customized Product Available | No |
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