Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Intended Use
Kanamycin Esculin Azide HiVeg Agar / Broth is used for isolation of Enterococci in foodstuffs.
Composition
| Ingredients | MV510 Grams/Litre | MV776 Grams/Litre |
|---|---|---|
| HiVeg hydrolysate | 20.00 | 20.00 |
| Yeast extract | 5.00 | 5.00 |
| Sodium chloride | 5.00 | 5.00 |
| Sodium citrate | 1.00 | 1.00 |
| Esculin | 1.00 | 1.00 |
| Ferric ammonium citrate | 0.50 | 0.50 |
| Sodium azide | 0.15 | 0.15 |
| Kanamycin sulphate | 0.02 | 0.02 |
| Agar | 12.00 | — |
Final pH (at 25°C) 7.0 ± 0.2
** Formula adjusted, standardized to suit performance parameters
Technical Specifications
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) | |
|---|---|---|
| Product Code | MV510/MV776 | M510/M776 |
| Hydrolysate | HiVeg hydrolysate | Casein enzymic hydrolysate |
Recommended for: Isolation of Enterococci in foodstuffs.
Reconstitution:
- (MV510): 44.67 g/l
- (MV776): 32.67 g/l
Quantity on preparation (100g):
- (MV510): 2.23 L
- (MV776): 3.06 L
pH (25°C): 7.0 ± 0.2
Supplement: None
Sterilization: 121°C / 15 minutes
Storage: Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Directions
Suspend 44.67 grams of MV510 or 32.67 grams of MV776 in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Warning: Sodium azide has a tendency to form explosive metal azides with plumbing materials. It is advisable to use enough water to flush off the disposables.
Principle and Interpretation
Kanamycin Esculin Azide HiVeg Media are prepared by using HiVeg hydrolysate which is free of BSE/TSE risks. These media are the modifications of Kanamycin Esculin Azide Medium which is formulated as per Mossel et al (1,2) to detect Enterococci in food stuffs. It can be used in the dip slide technique for bacteriological monitoring of foods like the conventional media used by Mossel et al (3).
HiVeg hydrolysate, yeast extract provides essential nutrients for Enterococci. Kanamycin sulphate and sodium azide are the selective inhibitory components. Esculin and ferric ammonium citrate together form the indicator system to detect esculin hydrolysing Enterococci forming black zones around the colonies.
Mossel et al (4) described the following procedure as - 1gm or 1ml mixed food is added to prechilled diluent (Tryptone water, HiVeg MV463) and decimal dilutions are prepared. The decimal dilutions are inoculated in Kanamycin Esculin Azide HiVeg Broth (MV776) and incubated at 35°C for 16-24 hours. If blackening of medium occurs, streaking is done on agar (MV510) and after incubation confirmatory tests are carried out.
Reaction
Reaction of 4.47% w/v of MV510 or 3.27% w/v of MV776 aqueous solution is pH 7.0 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35°C or 42°C for 18-24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery | Esculin hydrolysis |
|---|---|---|---|---|
| Enterococcus faecium (19434) | 103-105 | luxuriant | >70% | + |
| Enterococcus bovis (27960) | 103-105 | luxuriant | >70% | + |
| Escherichia coli (25922) | 103-105 | inhibited | 0% | — |
| Staphylococcus aureus (25923) | 103-105 | inhibited | 0% | — |
| Enterococcus faecalis (29212) | 103-105 | luxuriant | >70% | + |
Key : + = blackening of medium / black zone around the colony
Quality Control
Appearance of Powder: Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling: Firm, comparable with 1.2% Agar gel of MV510.
Colour and Clarity: Medium amber coloured, clear to slightly opalescent gel forms in petri plates, clear solution in tubes.
| Product Name | Kanamycin Esculin Azide HiVeg™ Broth |
|---|---|
| SKU | MV776 |
| Customized Product Available | No |
Write Your Own Review