Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Glucose HiVeg Peptone Agar is a highly nutritious medium used for cultivation of Agrobacterium and a wide variety of microorganisms. It is recommended for cultivation of a wide variety of microorganisms and Agrobacterium species.
Product Overview
This product, MV758, is a vegetable-based HiVeg peptone (Code MV). An animal-based alternative is M758 Peptic digest of animal tissue (Code M).
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone | 20.00 |
| Dextrose | 10.00 |
| Sodium chloride | 5.00 |
| Agar | 15.00 |
| Final pH (at 25°C) | 7.2 ± 0.2 |
Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 50 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
This medium is prepared by completely replacing animal based Peptic digest of animal tissue with HiVeg peptone and is the modification of Glucose Peptone Agar. Therefore it is free of TSE/BSE risks. It can also be used for cultivation of thermophilic organisms associated with flat sour spoilage in canned goods.
Glucose HiVeg Peptone Agar like the conventional Glucose Peptone Agar with addition of bromocresol purple (1% alcoholic solution) is suitable for cultivation of root nodulating bacteria (1). HiVeg peptone provides nitrogeneous nutrients especially amino acids and peptides. The presence of sodium chloride helps to maintain osmotic balance. Dextrose provides carbon and energy source. This medium supports growth of Agrobacterium species, as it grows abundantly in media containing dextrose as carbohydrate source (2).
Product Specifications
- Reconstitution: 50.0 g/l
- Quantity on preparation (500g): 10.0 L
- Supplement: None
- Storage: Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Quality Control
Appearance of Powder: Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling: Firm, comparable, with 1.5% Agar gel.
Colour and Clarity: Light yellow coloured, clear to slightly opalescent gel forms in petri plates.
Reaction: Reaction of 5.0% w/v aqueous solution is pH 7.2 ± 0.2 at 25°C.
Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Agrobacterium tumefaciens (23308) | 102-103 | good-luxuriant | > 70% |
| Escherichia coli (25922) | 102-103 | good-luxuriant | > 70% |
| Pseudomonas aeruginosa (27853) | 102-103 | good-luxuriant | > 70% |
| Staphylococcus aureus (25923) | 102-103 | good-luxuriant | > 70% |
| Enterococcus faecalis (29212) | 102-103 | good-luxuriant | > 70% |
References
- Subba Rao, N.S. 1977, Soil microorganisms and plant growth, Oxford and IBH publishing Co., India.
- Ronald M. Atlas (2004), Handbook of Microbiological Media, Lawrence C. Parks (ed.), 3rd Edition, CRC Press, p. No. 717.
| Product Name | Glucose HiVeg™ Peptone Agar |
|---|---|
| SKU | MV758 |
| Customized Product Available | No |
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