Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Esculin Azide HiVeg Broth is used for selective cultivation and identification of Streptococci.
Recommended for selective cultivation and identification of Streptococci.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone | 25.0 |
| Yeast extract | 5.0 |
| Synthetic detergent | 5.0 |
| Sodium citrate | 1.0 |
| Esculin | 1.0 |
| Ferric ammonium citrate | 0.5 |
| Sodium azide | 0.25 |
Final pH (at 25°C) 7.2 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 37.8 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Warning: Sodium Azide has a tendency to form explosive metal azides with plumbing materials. It is advisable to use enough water to flush off the disposables.
Principle and Interpretation
This medium is prepared by using HiVeg peptone and synthetic detergent, which make the medium free of BSE/TSE risks. Rochaix (1) noted the importance of esculin hydrolysis in the identification of Enterococci. Isenberg et al modified this medium by adding Sodium azide. Esculin Azide HiVeg Broth is the modification of the medium prepared by Isenberg (2). The broth is selective due to presence of synthetic detergent and sodium azide and provides rapid growth of Streptococci.
HiVeg peptone and Yeast extract provide nitrogenous nutrients to the organisms. Synthetic detergent inhibit other gram-positive bacteria while sodium azide inhibits gram-negative bacteria. Streptococci hydrolyze esculin to esculetin and dextrose. Esculetin and Ferric ammonium citrate forms dark brown to black complex, imparting dark brown colour to the broth.
Technical Data
| Reconstitution | 37.8 g/l |
| Quantity on preparation (500g) | 13.22 L |
| pH (25°C) | 7.2 ± 0.2 |
| Supplement | None |
| Sterilization | 121°C / 15 minutes. |
Storage and Shelf Life
Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Cultural Response
Cultural characteristics observed after an incubation at 35 -37°C for 18 - 24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Esculin Hydrolysis |
|---|---|---|---|
| Enterococcus faecalis (29212) | 102-103 | good to luxuriant | + |
| Escherichia coli (25922) | 102-103 | inhibited | - |
| Streptococcus bovis (27960) | 102-103 | good to luxuriant | + |
| Streptococcus pyogenes (19615) | 102-103 | poor to good | - |
Quality Control
Appearance of powder
Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Colour and Clarity
Amber coloured, clear solution having purplish tinge.
Reaction
Reaction of 3.78% w/v aqueous solution is pH 7.2 ± 0.2 at 25°C.
References
- Rochaix 1924, C.R. Soc. Biol 90:771.
- Isenberg, 1970, Clin. Lab. Forum.
| Product Name | Esculin Azide HiVeg™ Broth |
|---|---|
| SKU | MV749 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Isenberg, 1970, Clin. Lab. Forum. 2.Swan A., 1954, J. Clin. Pathol., 7:160 3.Facklam R. R. and Moody M. D., 1970, Appl.Microbiol., 20:2451. Isenberg, 1970, Clin. Lab. Forum. 2.Swan A., 1954, J. Clin. Pathol., 7:160 3.Facklam R. R. and Moody M. D., 1970, Appl. Microbiol., 20:245 |
| Customized Product Available | No |
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