Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for cultivation and identification of fastidious anaerobic bacteria.
Composition
| Ingredients | g/L |
|---|---|
| HiVeg™ hydrolysate | 5.000 |
| Yeast extract | 5.000 |
| Sodium chloride | 2.500 |
| Sodium sulphite | 0.100 |
| L-Cystine | 0.400 |
| L-Tryptophan | 0.200 |
| Vitamin K1 | 0.010 |
| Ferric pyrophosphate | 0.010 |
| Agar | 20.000 |
| Final pH (at 25°C) | 7.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 33.22 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Organisms that grow in the absence of oxygen are termed as anaerobes. Depending upon their ability to tolerate oxygen, they are classified as either facultative or obligate anaerobes. The anaerobic gram-negative bacteria are part of the normal flora of the upper respiratory tract, mouth, intestinal tract and urinogenital tract of human and animals. The bile-resistant Bacteroides fragilis group is the most commonly recovered anaerobe in clinical specimens and is more resistant to antimicrobial agents than any other anaerobe. Fusobacterium necrophorum is a very virulent anaerobe that may cause severe infections, usually in children or young adults (1).
L.D. Medium or Lombard-Dowell Medium was developed by Dowell and Lombard (2) for the cultivation and identification of fastidious anaerobic bacteria. L.D. Agar is used to evaluate the degree of growth of anaerobes and also to assess indole and catalase production by Bacteroides and Fusobacterium species.
L.D. Agar is essentially a casein digest agar enriched with hemin, vitamin K1, L-cystine and yeast extract (3). This medium contains various nutritious substances, which can promote the growth of fastidious anaerobic bacteria. L.D. HiVeg™ Agar is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks. HiVeg™ hydrolysate and yeast extract provide the necessary nitrogenous nutrients while Ferric pyrophosphate and vitamin K1 supply additional growth factors. L-cystine and L-tryptophan serve as the amino acid sources. Sodium sulphite is an antioxidant. Sodium chloride maintains osmotic balance of the medium. Catalase-positive reaction may not be evident uptill 30 seconds to 1 minute after application of 3% hydrogen peroxide (4,5).
Type of specimen
Please add specimens
Specimen Collection and Handling
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Catalase-positive reaction may not be evident uptill 30 seconds to 1 minute after application of 3% hydrogen peroxide (4,5).
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 2.0% agar gel.
Colour and Clarity of prepared medium
Medium amber coloured clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 3.32% w/v aqueous solution at 25°C. pH: 7.4±0.2
pH
7.20-7.60
Cultural Response
Cultural characteristics observed under anaerobic condition, after an incubation at 35-37°C for 40-48 hours.
| Organism | Growth | Indole production | Catalase |
|---|---|---|---|
| Bacteroides fragilis ATCC 25285 | good-luxuriant | negative reaction | positive reaction |
| Bacteroides corrodens | fair-good | negative reaction | negative reaction |
| Fusobacterium necrophorum ATCC 25286 | good-luxuriant | positive reaction | negative reaction |
| Fusobacterium nucleatum ATCC 25586 | fair to good | positive reaction | negative reaction |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (6,7).
| Product Name | L.D. HiVeg™ Agar |
|---|---|
| SKU | MV742 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Dowell V. and Lombard G., June 1977, U.S., DHEW, Center for Disease Control (CDC), Atlanta. Ga. 2.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. I, Williamsand Wilkins, Baltimore 3.Finegold S. M., Baron E. J., Bailey and Scotts Diagnostic Microbiology, 8th Ed., 1990, The C.V. Mosby Company 4.Koneman E., Allen S., Dowell V. and Sommers H., 1979, Colour Atlas and Textbook of Diagnostic Microbiology, J. B.Lippincott Co., Philadelphia. 5.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,8th Ed., American Society for Microbiology, Washington, D.C. |
| Customized Product Available | No |
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