Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Oak Wilt Fungus HiVeg Agar is used for cultivation of Oak Wilt Fungus.
Composition
| Ingredients | Grams/Litre |
|---|---|
| Malt extract | 22.0 |
| HiVeg peptone No. 4 | 8.0 |
| Synthetic detergent No. II | 5.0 |
| Agar | 15.0 |
Final pH (at 25°C) 5.7 ± 0.2
Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 50 grams in 1000 ml distilled water. Heat to boiling to dissolve completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
This medium is developed by using HiVeg peptone No. 4 and Synthetic detergent No. II, which are free from BSE/ TSE risks associated with animal based peptones. Oak Wilt Fungus HiVeg Agar is modification of Oak Wilt Fungus Agar developed by Gallway and Bergers (1) and used for cultivation of Oak Wilt Fungus. Oak wilt disease is caused by a fungus Ceratocystis fagacearum. After infection by this fungus, the trees contract oak wilt and die and the oak wilt fungus forms fungal mats under the bark of these dead trees.
Oak Wilt Fungus HiVeg Agar supports good growth of this fungus. Malt extract solids and HiVeg peptone No.4 provides necessary nutrients required for metabolism by fungus. Typical morphology and pigmentation of fungus can be studied. The acidic pH of medium favours the growth of fungus.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV669 | M669 |
| HiVeg peptone No.4 | Mycological peptone |
| Synthetic detergent No.Il | Oxgall |
| Recommended for | Cultivation of Oak Wilt Fungus |
|---|---|
| Reconstitution | 50.0 g/l |
| Quantity on preparation (100g) | 2.0 L |
| pH (25°C) | 5.7 ± 0.2 |
| Supplement | None |
| Sterilization | 121°C / 15 minutes |
| Storage | Dry Medium-Below 30°C, Prepared Medium 2-8°C. |
Quality Control
Appearance of powder
Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel.
Colour and Clarity
Yellow coloured, clear to slightly opalescent gel forms in petri plates.
Reaction
Reaction of 5.0% w/v aqueous solution is pH 5.7 ± 0.2 at 25°C
Cultural Response
Cultural characteristics observed after an incubation at 25-30°C for 48-72 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Aspergillus niger (16404) | good-luxuriant |
| Ceratocystis fagacearum | good-luxuriant |
| Saccharomyces cerevisiae (9763) | good-luxuriant |
References
- Gallway L D and Bergers R., 1952, Applied Mycology and Bacteriology; 3rd ed., Leronard Hill., London pg. 54 and 57.
| Product Name | Oak Wilt Fungus HiVeg™ Agar |
|---|---|
| SKU | MV669 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Gallway L. D. and Burgers R., 1952, Applied Mycology and Bacteriology; 3rd Ed., Leronard Hill., London pg. 54 and 57. |
| Customized Product Available | No |
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Oak Wilt Fungus Agar
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₹2,295.00