Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Phenolphthalein Phosphate HiVeg Agar is recommended for identification of phosphatase positive colonies of Staphylococcus aureus.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone | 5.0 |
| HiVeg extract | 3.0 |
| Sodium chloride | 5.0 |
| Sodium phenolphthalein phosphate | 0.012 |
| Agar | 15.0 |
Final pH (at 25°C) 7.4 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 28 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
This medium is prepared by using HiVeg peptone and HiVeg extract in place of Peptic digest of animal tissue and Beef extract which make the medium free of BSE/TSE risks. Phenolphthalein Phosphate HiVeg Agar is the modification of Phenolphthalein Phosphate Agar which is used for the identification of phosphatase positive colonies of Staphylococccus aureus which is a coagulase positive pathogenic strain (1). Phosphatase production is determined by the liberation of phenolphthalein which is indicated by the change in colour of the medium (2). When alkali is added to this medium, the liberated phenolphthalein gives bright pink - red colouration. HiVeg peptone and HiVeg extract supply the nitrogenous compounds, growth factors and trace ingredients essential for the growth of Staphylococcus aureus. Sodium phenolphthalein phosphate serves as a substrate for the phosphatase enzyme. Sodium chloride maintains osmotic equilibrium.
Product Profile
| Vegetable based (Code MV) © | Animal based (Code M) |
|---|---|
| MV652 | M652 |
| HiVeg peptone | Peptic digest of animal tissue |
| HiVeg extract | Beef extract |
Recommended for
Identification of phosphatase positive colonies of Staphylococcus aureus.
Reconstitution
28.0 g/l
Quantity on preparation
(500g): 17.85 L
(100g): 3.57 L
pH (25°C)
7.4 ± 0.2
Supplement
None
Sterilization
121°C / 15 minutes.
Storage
Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Gelling
Firm, comparable with 1.5% Agar gel.
Colour and Clarity
Yellow coloured, clear gel forms in tubes as slants.
Reaction
Reaction of 2.8% w/v aqueous solution is pH 7.4 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Phosphatase* |
|---|---|---|---|
| Escherichia coli (25922) | 10²-10³ | luxuriant | - |
| Staphylococcus aureus (25923) | 10²-10³ | luxuriant | + |
| Staphylococcus epidermidis (12228) | 10²-10³ | luxuriant | + |
- Key : * = after incubation add 1 drop of 40% NaOH
- + = Bright pink-red colouration after addition of 40% NaOH
Quality Control
Appearance of powder
Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
| Product Name | Phenolphthalein Phosphate HiVeg™ Agar |
|---|---|
| SKU | MV652 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Easmon C. S. F., Adlam C., 1983, Staphylococci and staphylococcal infections. Vol. 1 and 2, Academic Press, London, 2.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williamsand Wilkins, Baltimore. 3.Lewis B., 1961, J. Med. Lab. Technol., 18 : 112. 4.Barber M. and Kuper S. W. A., 1951, J. Pathol. Bacteriol., 63:65. |
| Customized Product Available | No |
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