Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for cultivation of Staphylococci.
Composition
| Ingredients | g/L |
|---|---|
| HiVeg™ peptone | 10.000 |
| Yeast Extract | 5.000 |
| Sodium chloride | 5.000 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 20.0 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Dispense in tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Staphylococcus Broth is recommended for the cultivation of Staphylococci from clinical and nonclinical specimens. It is a nutritive medium providing enough nutrients for the growth of Staphylococci. Standard Staphylococcus HiVeg™ Broth is prepared by completely replacing animal based peptone with vegetable peptones to avoid BSE/TSE risks associate with animal peptones. HiVeg™ peptone and yeast extract supply essential growth factors such as nitrogen, carbon, sulphur, vitamins and trace nutrients. Sodium chloride maintains the osmotic balance of the medium.
Type of specimen
Food samples; Water samples
Specimen Collection and Handling
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (1). For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(2) After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- 1. Further biochemical and serological tests must be carried out for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Yellow coloured homogeneous free flowing powder
Colour and Clarity of prepared medium
Light amber coloured clear solution without any precipitate.
Reaction
Reaction of 2% w/v aqueous solution at 25°C. pH: 7.0±0.2
pH
6.80-7.20
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.
| Organism | Growth |
|---|---|
| Staphylococcus aureus ATCC 19095 |
good - luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) |
good - luxuriant |
| Staphylococcus epidermidis ATCC 12228 (00036*) |
good - luxuriant |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Standard Staphylococcus HiVeg™ Broth |
|---|---|
| SKU | MV578 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| Customized Product Available | No |
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