Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Cholera HiVeg Medium Base is a selective medium used for the selective isolation of Vibrio species from specimens grossly contaminated with Enterobacteriaceae.
Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone | 10.0 |
| HiVeg extract | 10.0 |
| Sucrose | 10.0 |
| Sodium lauryl sulphate | 0.1 |
| Sodium chloride | 20.0 |
| Sodium carbonate | 5.0 |
| Agar | 10.0 |
Final pH (at 25°C) 8.5 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 65.1 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Cool to 70°C and add 2 ml of a sterile 1% Potassium Tellurite solution (FD052) and 5 ml of sterile defibrinated blood. Maintain at 70°C for a few minutes. Cool to 50°C and pour into sterile petri plates.
Principle and Interpretation
Cholera HiVeg Medium Base is prepared by using HiVeg peptone and HiVeg extract which are free of BSE/TSE risks associated with animal based peptones. Cholera HiVeg Medium Base is the modification of Cholera Medium Base which is based on the formulation described by Felsenfeld and Watanabe (1) for the isolation of Vibrio cholerae and similar Vibrios from specimens contaminated with Enterobacteriaceae. It is a selective medium used for the isolation of Vibrio species from specimens contaminated with enteric bacteria.
HiVeg extract and HiVeg peptone provide nitrogenous nutrients whereas sucrose serves as the fermentable carbohydrate source for the metabolism of Vibrios. Added blood provides extra nutrition to growing Vibrios. Sodium lauryl sulphate inhibits many contaminating organisms. Potassium tellurite is a selective and differentail agent. It inhibits many gram -positive bacteria and is also reduced by Vibrio form grey to black coloured colonies. Alkaline pH of medium and sodium chloride selctively favours growth of Vibrio species.
Product Profile
| Vegetable based (Code MV) | Animal based (Code M) |
|---|---|
| MV558 | M558 |
| HiVeg peptone | Peptic digest of animal tissue |
| HiVeg extract | Beef extract |
Recommended for
: Isolation of Vibrio species from specimens grossly contaminated with Enterobacteriaceae.
Reconstitution
| Quantity on preparation (500g) | : 65.1 g/l |
| : 7.68 L | |
| pH (25°C) | : 8.5 ± 0.2 |
| Supplement | : 1% Potassium Tellurite Solution (FD052), Defribrinated blood |
| Sterilization | : Boiling (DO NOT AUTOCLAVE) |
| Storage | : Dry Medium - Below 30°C, Prepared Medium 2 - 8°C. |
Quality Control
Appearance of powder
Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.0% Agar gel.
Colour and Clarity
Basal medium forms yellow coloured slightly opalescent gel. With addition of Potassium tellurite and blood upon heating brownish red opalescent gel forms in petri plates.
Reaction
Reaction of 6.5% w/v aqueous solution is pH 8.5 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18 - 24 hours with added 1% Potassium Tellurite Solution (FD052) and sterile defibrinated blood.
Organisms (ATCC)
| Organisms (ATCC) | Inoculum (CFU) | Growth | Colour of colonies | Recovery |
|---|---|---|---|---|
| Bacillus subtilis (6633) | 10²-10³ | inhibited | - | 0% |
| Escherichia coli (25922) | 10²-10³ | inhibited | - | 0% |
| Proteus mirabilis (25933) | 10²-10³ | inhibited | - | 0% |
| Pseudomonas aeruginosa (27853) | 10²-10³ | inhibited | - | 0% |
| Vibrio cholerae (15748) | 10²-10³ | luxuriant | grey | >50% |
| Vibrio parahaemolyticus (17802) | 10²-10³ | luxuriant | light grey | >50% |
| Product Name | Cholera HiVeg™ Medium Base |
|---|---|
| SKU | MV558 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Felsenfeld O. and Watanabe Y., 1958, U.S. Armed Forces Med. J., 9 (7) : 975. |
| Customized Product Available | No |
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