Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Loeffler HiVeg™ Medium Base
Used for the cultivation of Corynebacterium diphtheriae from clinical specimens and in pure cultures,detection of chromogenesis,proteolysis and the production of ascospores.
Your enquiry has been submitted
Please login to Post an Enquiry.
Intended Use
Recommended for cultivation of Corynebacterium diphtheriae from clinical specimens and in pure cultures, detection of chromogenesis, proteolysis and the production of ascospores.
Composition**
| Ingredients | g/L |
|---|---|
| HiVeg™ special peptone | 2.500 |
| HiVeg™ extract | 2.500 |
| Sodium chloride | 1.250 |
| Dextrose (Glucose) | 2.500 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 8.75 grams in 250 ml purified / distilled water. Dissolve the medium completely and sterilize by autoclaving at 10 lbs pressure (115°C) for 20 minutes. Cool to 50-55°C and aseptically add 750 ml of sterile horse serum (RM1239). Mix well and aseptically dispense into sterile tubes. Sterilize the medium by inspissation at 80-85°C for 2 hours in free flowing steam for atleast 3 consecutive days.
Principle And Interpretation
Loeffler Medium was originally devised by Loeffler (1) and was further modified by Perry and Petran (2) and Buck (3). Loeffler HiVeg™ Medium Base enhances primary and secondary isolation and cultivation of fastidious pathogenic microorganisms especially from nose and throat. It also restores virulence and other identifying properties (microscopic and colonial) after they have been lost due to prolonged incubation or repeated subculturing. Loeffler HiVeg™ Medium Base is prepared by completely replacing animal based peptone with vegetable peptones to avoid BSE/TSE risks associate with animal peptones. The high serum content helps in determining proteolytic activity of organisms. It is also used for demonstration of pigmentation and ascospores. HiVeg™ special peptone, HiVeg™ extract provide essential growth nutrients. Dextrose is the source of fermentable carbohydrate and energy. Rub the swabs directly over the surface of medium and after incubation; prepare the smears from surface of slope. For proteolysis testing, inoculate slant and prior to incubation, flood the slant with Brewer Thioglycollate HiVeg™ Medium (MV019). Incubation should be carried out for 3-4 days or much longer for appearance of proteolysis. Loeffler HiVeg™ Medium Base should be used in parallel with Serum Tellurite Agar for selective isolation of Corynebacteria (4).
Type of specimen
Clinical samples- a nasopharyngeal and throat swabs
Specimen Collection and Handling:
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions:
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- For optimum recovery of C. diphtheriae, a nasopharyngeal and throat swab should be obtained while collection of specimen.
- Further biochemical and serological tests need to be carried out for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Basal: Light amber coloured clear to slightly opalescent solution. After addition of horse serum: Off-white coloured opaque solution
Reaction
Reaction of 3.52% w/v aqueous solution of base at 25°C. pH: 7.3±0.2
pH
7.10-7.50
Cultural Response
Cultural characteristics observed with added 750ml horse serum, after an incubation at 35-37°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Corynebacterium diphtheriae ATCC 11913 | 50-100 | fair-good |
| Pseudomonas aeruginosa ATCC 10145 (00024*) | 50-100 | good (green colonies with proteolysis) |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | good (yellow to gold colonies) |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Loeffler HiVeg™ Medium Base |
|---|---|
| SKU | MV537 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.Loeffler F., 1887, Zentralb. Bakteriol. Parasitenkd., 2:102. 2.Perry and Petran, 1939, J. Lab. Clin. Med., 25:71. 3.Buck, 1949, J. Lab. Clin. Med., 34:582. 4.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Maintenance of Medical Bacteria, Vol. I, Williams and Wilkins,Baltimore. |
| Customized Product Available | No |
Write Your Own Review
Horse serum
As low as
₹5,326.00
Loeffler Serum Medium Base
As low as
₹1,569.00
Mueller Tellurite Agar Base
As low as
₹6,531.00
Tinsdale Agar Base
As low as
₹6,720.00
Hartley’s Digest Broth
As low as
₹1,310.00
Dextrose Proteose Peptone Agar Base
As low as
₹5,989.00
Upsell Products
L.M. Slant (Loeffler Medium)
As low as
₹2,112.00