Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for enrichment of Streptococci (Enterococcus faecalis) from various samples.
Composition
| Ingredients | g/L |
|---|---|
| HiVeg™ hydrolysate | 26.000 |
| Yeast extract | 6.000 |
| Synthetic detergent No.2 | 3.000 |
| Sodium chloride | 5.000 |
| Sodium citrate | 1.000 |
| Esculin | 1.000 |
| Ferric ammonium citrate | 0.500 |
| Sodium azide | 0.250 |
| Final pH (at 25°C) | 7.0±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 42.8 grams in 1000 ml purified/distilled water. Heat to dissolve the medium completely. Dispense in 9 ml amounts into test tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 20 minutes. Cool to 45-50°C.
Principle And Interpretation
The ability of Enterococci to hydrolyze the esculin was first observed by Rochaix (1). The Enterococci can hydrolyze the esculin but not the other Streptococci can do it. Presumptive identification of group D Streptococci by bile esculin test was reported by Facklam and Moody (2). Later on Bile Esculin medium was modified by Isenberg et al (3) by reducing the bile concentration and by adding sodium azide to the medium.
Streptococcus Enrichment HiVeg™ Broth (SE HiVeg™ Broth) is prepared by completely replacing animal based peptone with vegetable peptones to avoid BSE/TSE risks associate with animal peptones. HiVeg™ hydrolysate and yeast extract provide nitrogenous compounds, carbon, sulphur, trace elements and vitamin B complex, essential for Streptococci. Esculin is hydrolyzed by group D Streptococci (including Enterococci) to esculetin and dextrose. Esculetin reacts with ferric ammonium citrate to form a dark brown-black coloured complex (4). Synthetic detergent No.2 inhibits gram-positive bacteria other than Streptococci. Sodium azide inhibits gram-negative bacteria.
Type of specimen
Food and dairy samples; Water samples
Specimen Collection and Handling
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (5,6,7). For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(8) After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Further biochemical and serological tests must be carried out for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Yellow coloured homogeneous free flowing powder
Colour and Clarity of prepared medium: Light amber coloured clear solution with a bluish tinge.
Reaction: Reaction of 4.28% w/v aqueous solution at 25°C. pH: 7.0±0.2
pH: 6.80-7.20
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 18-48 hours
| Organism | Growth | Colour of medium |
|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | inhibited | - |
| Enterococcus faecalis ATCC 29212 (00087*) | good-luxuriant | black |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | inhibited | - |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (9,10).
| Product Name | Streptococcus Enrichment HiVeg™ Broth (SE HiVeg™ Broth) |
|---|---|
| SKU | MV465 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.Rochaix, 1924, C.R. Soc. Biol., 90:771. 2.Facklam and Moody, 1970, Appl. Microbiol., 20:245. 3.Isenberg, Goldberg and Sampson, 1970, Appl. Microbiol., 20:433. 4.MacFaddin J., 1980, Biochemical Tests for Identification of Medical Bacteria, 2nd ed., Williams and Wilkins, Baltimore. |
| Customized Product Available | No |
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