Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition
| Ingredients | Grams/Litre |
|---|---|
| HiVeg peptone | 5.0 |
| Yeast extract | 3.0 |
| Agar | 15.0 |
Final pH (at 25°C) 7.2 ± 0.2
** Formula adjusted, standardized to suit performance parameters.
Product Profile
| Vegetable based (Code MV)® | Animal based (Code M) |
|---|---|
| MV456 | M456 |
| HiVeg peptone | Peptic digest of animal tissue |
Recommended for: Plate count of microorganisms in water.
Reconstitution: 23.0 g/l
Quantity on preparation (500g): 21.73 L
Quantity on preparation (100g): 4.34 L
pH (25°C): 7.2 ± 0.2
Supplement: None
Sterilization: 121°C / 15 minutes.
Storage: Dry Medium - Below 30°C, Prepared Medium 2 - 8°C.
Directions
Suspend 23 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
This medium is prepared by using HiVeg peptone in place of Peptic digest of animal tissue which makes the medium BSE/TSE free. Yeast Extract HiVeg Agar is the modification of Yeast Extract Agar which is formulated according to the formula described by Windle Taylor (1) for the plate count of microorganisms in water.
Yeast extract and HiVeg peptone provide nitrogenous compounds, vitamin B complex and other growth nutrients. Organisms forming visible colonies after 24 hrs at 35°C are enumerated seperately from those forming visible colonies after 3 days at 20-22°C. (2)
Quality Control
Appearance of powder: Light yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling: Firm, comparable with 1.5% Agar gel.
Colour and Clarity: Yellow coloured, clear to slightly opalescent gel forms in petri plates.
Reaction: Reaction of 2.3% w/v aqueous solution is pH 7.2 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 18 - 24 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Enterobacter aerogenes (13048) | 102-103 | luxuriant | >70% |
| Escherichia coli (25922) | 102-103 | luxuriant | >70% |
| Pseudomonas aeruginosa (27853) | 102-103 | luxuriant | >70% |
| Staphylococcus aureus (25923) | 102-103 | luxuriant | >70% |
| Product Name | Yeast Extract HiVeg™ Agar |
|---|---|
| SKU | MV456 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1.Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and Wastewater,23rd ed., APHA, Washington, D.C. 2.Dept. of Health and Social Security, 1982, report No.71: HMSO, London, 54. 3.Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition. 4.Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock.,D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1. 5.Taylor W. E., 1958, The Examination of Waters and Water Supplies, 7th Ed., Churchill Ltd, London, pg. 394, 778. |
| Customized Product Available | No |
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