Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for mass cultivation of Treponema pallidum, Reiter strain for antigen production and other studies.
Composition
| Ingredients | g/L |
|---|---|
| HiVeg™ hydrolysate | 15.000 |
| Dextrose (Glucose) | 5.000 |
| Yeast extract | 5.000 |
| Sodium chloride | 2.500 |
| Sodium thioglycollate | 0.500 |
| L-Cystine hydrochloride | 1.000 |
| Final pH (at 25°C) | 7.1±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 29.0 grams in 1000 ml purified/distilled water. Add 0.25 grams of TEM-4TR-Diacetyl Tartaric Acid Ester of Monoglycerides of Animal Origin (TEM-4TR) if desired. Heat with frequent stirring and boil for 1 minute. Dispense in test tubes filling them half full (about 15-20 ml in 6" inch tubes). If bigger containers are used, maintain the surface to volume ratio similar to that of tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and add sterile inactivated 10% v/v sheep/rabbit bovine serum.
Principle And Interpretation
The general term spirochaete is often used to embrace Treponema species and organisms similar to spiral morphology. Spirolate Broth, OMATA medium was formulated by Omata and Disraely (1) for cultivating oral Fusobacteria. It is used for the mass cultivation of Reiter treponemes in a medium without agar for antigen production and other studies. It can also be used for the cultivation of other Spirochetes. Supplementation with fatty acids enhances the growth of Reiter Treponema. Spirolate HiVeg™™ Broth, OMATA is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks. HiVeg™™ hydrolysate, yeast extract provide nitrogenous growth factors, minerals and vitamin B complex for the growth of Reiter treponemes. Dextrose serves as the carbon source. Sodium chloride maintains osmotic equilibrium of the medium. Thioglycollate minimizes the oxygen tension, which is optimum for the growth of treponemes. L-cystine hydrochloride is a reducing agent and is less toxic to Fusobacteria (1). The addition of TEM-4TR provides fatty acids, which enhances the growth of Reiter treponemes (2).
Type of specimen
Isolated Microorganism
Specimen Collection and Handling
Inoculate Spirolate Broth with 0.05 ml aliquots of a 7 days pure culture in Thioglycollate Medium without indicator, supplemented with 10% inactivated sheep, rabbit or bovine serum. Incubate for minimum 7 days at 35-37°C in an anaerobic atmosphere.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- It is adviced to use 7 days pure culture to avoid erroneous results.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light straw coloured clear to slightly opalescent solution
Reaction
Reaction of 2.9% w/v aqueous solution at 25°C. pH: 7.1±0.2
pH
6.90-7.30
Cultural Response
Cultural characteristics observed with added 10% inactivated sheep/rabbit bovine serum after an incubation at 35-37° C for minimum 7 days under anaerobic conditions.
| Organism | Growth |
|---|---|
| Treponema pallidum (Reiter strain) | good-luxuriant |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Spirolate HiVeg™ Broth, OMATA |
|---|---|
| SKU | MV412 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Omata R. R. and Disraely M. N., 1956, J. Bacteriol., 72:677. 2.Power D. A. and Pelczar M. J., 1959, J. Bacteriol., 77 : 789 |
| Customized Product Available | No |
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Spirolate Broth, OMATA
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